A gold@polydopamine core–shell nanoprobe for long-term intracellular detection of microRNAs in differentiating stem cells
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AbstractMicroRNAs (miRNAs) represent an emerging class of biomarkers for studying and understanding biological events; the development of viable tools for detecting or monitoring the intracellular expression levels of specific miRNAs is of great interest to life scientists and biomedical engineers. Here, we describe the fabrication of a novel class of core–shell nanoprobes that comprise a gold nanoparticle core and a polydopamine (PDA) shell. Our nanoprobes can be used to specifically track the expression profiles of two miRNA markers of osteogenic differentiation (i.e., osteogenesis), namely, miR-29b and miR-31, in differentiating human mesenchymal stem cells (hMSCs). The newly designed nanoprobes may hold great promise in the noninvasive investigation of the long-term dynamics of cellular events such as stem cell differentiation.MicroRNAs (miRNAs) represent an emerging class of biomarkers for studying and understanding biological events; the development of viable tools for detecting or monitoring the intracellular expression levels of specific miRNAs is of great interest to life scientists and biomedical engineers. Here, we describe the fabrication of a novel class of core–shell nanoprobes that comprise a gold nanoparticle core and a polydopamine (PDA) shell. Our nanoprobes can be used to specifically track the expression profiles of two miRNA markers of osteogenic differentiation (i.e., osteogenesis), namely, miR-29b and miR-31, in differentiating human mesenchymal stem cells (hMSCs). The newly designed nanoprobes may hold great promise in the noninvasive investigation of the long-term dynamics of cellular events such as stem cell differentiation.
All Author(s) ListCHOI Chun Kit, CHOI Chung Hang Jonathan, BIAN Liming
All Editor(s) ListSarah Hurst Petrosko, Emily Day
EditionSecond
Book titleBiomedical Nanotechnology: Methods and Protocols
Series TitleMethods in Molecular Biology
Year2017
PublisherSpringer
Pages155 - 164
ISBN978-1-4939-6840-4
LanguagesEnglish-United States
KeywordsmicroRNAs, intracellular detection, gold nanoparticles, polydopamine shell, osteogenic differentiation, stem cells

Last updated on 2018-20-01 at 18:17