Protocadherin20 Acts as a Tumor Suppressor Gene: Epigenetic Inactivation in Nasopharyngeal Carcinoma
Publication in refereed journal

香港中文大學研究人員

引用次數
替代計量分析
.

其它資訊
摘要Genetic alterations of 13q21 (PCDH 8,9,17, and 20) are frequently observed in multiple tumors, suggesting the presence of critical tumor suppressor genes (TSGs). Protocadherin20 (PCDH20), located at 13q21.2, belongs to the 1-protocadherins, which constitutes one of the largest subgroup within the adherin superfamily. Frequent downregulation/silencing of PCDH20 was found in NPC cell lines using semiquantitative PCR. PCDH20 mRNA was broadly expressed in normal nasopharyngeal tissues and cell lines. Promoter methylation of PCDH20 was observed in 80% (4/5) of NPC cell lines and 78.4% (40 of 51) of primary tumors by methylation-specific PCR, but rarely in normal nasopharygeal tissues and nasopharyngeal epithelial cell line (NP69). The silencing of PCDH20 can be reversed by pharmacological demethylation, indicating an epigenetic mechanism. Restoration of PCDH20 expression in NPC cells strongly suppressed cell numbers and colony formation. Overexpression of PCDH20 antagonized Wnt/-catenin signaling pathway and promoted -catenin to translocate from nucleus to cytoplasm and membrane. PCDH20 significantly inhibited the migration and invasion ability of NPC cells. PCDH20 also inhibited epithelial-mesenchymal transition (EMT) through enhanced expression of E-cadherin. Our study identified PCDH20 as a functional tumor suppressor and an important antagonist of Wnt/-catenin signaling and EMT, with frequent epigenetic inactivation in NPC. J. Cell. Biochem. 116: 1766-1775, 2015. (c) 2015 Wiley Periodicals, Inc.
著者Chen T, Long BL, Ren GS, Xiang TX, Li LL, Wang ZH, He Y, Zeng Q, Hong SL, Hu GH
期刊名稱Journal of Cellular Biochemistry
出版年份2015
月份8
卷號116
期次8
出版社WILEY-BLACKWELL
頁次1766 - 1775
國際標準期刊號0730-2312
電子國際標準期刊號1097-4644
語言英式英語
關鍵詞-CATENIN; EPIGENETIC INACTIVATION; EPITHELIAL-MESENCHYMAL TRANSITION; METHYLATION; NASOPHARYNGEAL CARCINOMA; PROTOCADHERIN 20; TUMOR SUPPRESSOR GENE; Wnt
Web of Science 學科類別Biochemistry & Molecular Biology; Cell Biology

上次更新時間 2020-01-12 於 01:21