Species variability in the expression of met- and leu-enkephalin-like immunoreactivity in mammalian Merkel cell dense-core granules - A light- and electron-microscopic immunohistochemical study
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AbstractImmunogold staining failed to show met-enkephalin immunoreactivity in the Merkel cell dense-core granules of rats when examined by electron microscopy, but showed gold particle staining in the Merkel cell dense-core granules of mice and nude mice. Merkel cells of hamster, guinea pig, rabbit, cat and dog were also examined using a similar method, and different antisera dilutions. Immunogold particles were consistently found in the dense-core granules of mice and nude mice at all antisera dilutions, but not in the other species, except in the dog, where a very low labelling response was encountered. Merkel cells from skin touch domes or sinus hair follicles, did not exhibit any difference in peptide expression as far as met-enkephalin immunoreactivity was concerned. In addition, all species studied, including mice and nude mice, did not show leu-enkephalin immunoreactivity in their Merkel cell dense-core granules. It is concluded that species variability in peptide expression occurs in the Merkel cell dense-core granules, and may be closely related to the different methodologies used. © 1992 Springer-Verlag.
All Author(s) ListCheng Chew S.B., Leung P.Y.
Journal nameCell and Tissue Research
Year1992
Month8
Day1
Volume Number269
Issue Number2
PublisherSpringer Verlag
Place of PublicationGermany
Pages347 - 351
ISSN0302-766X
LanguagesEnglish-United Kingdom
KeywordsDense-core granules, Enkephalin, Immunocytochemistry, Mammalian species, Merkel cells, Skin, Touch dome

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