Conjugation of ϕBT1-derived integrative plasmid pDZL802 in Amycolatopsis mediterranei U32
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要The genus Amycolatopsis is well known for its ability to produce antibiotics, and an increasing number of valuable biotechnological applications, such as bioremediation, biodegradation, bioconversion, and potentially biofuel, that use this genus have been developed. Amycolatopsis mediterranei is an industrial-scale producer of the important antibiotic rifamycin, which plays a vital role in antimycobacterial therapy. Genetic studies of Amycolatopsis species have progressed slowly due to the lack of efficient transformation methods and stable plasmid vectors. In A. mediterranei U32, electroporation and replicable plasmid vectors have been developed. Here, we establish a simple and efficient conjugal system by transferring integrative plasmid pDZL802 from ET12567 (pUZ8002) to A. mediterranei U32, with an efficiency of 4 × 10(-5) CFU per recipient cell. This integrative vector, based on the ϕBT1 int-attP locus, is a stable and versatile tool for A. mediterranei U32, and it may also be applicable to various other Amycolatopsis species for strain improvement, heterologous protein expression, and synthetic biology experiments.
著者Li, Zhou, Wang, Zhao, Ding
期刊名稱Bioengineered
出版年份2017
卷號8
期次5
出版社Taylor & Francis
頁次549 - 554
國際標準期刊號2165-5979
電子國際標準期刊號2165-5987
語言英式英語

上次更新時間 2020-20-11 於 02:36