Reversal of TNF-α resistance by hyperthermia: Role of mitochondria
Publication in refereed journal

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其它資訊
摘要The aim of this study is to examine the effect of hyperthermia on tumour necrosis factor-α (TNF-α) resistance in L929-11E cells. L929-11E is a TNF-α resistant variant derived from L929 cells, a commonly used model for TNF-α study. Based on the results from flow cytometry and Western blotting, hyperthermia (43°C, 3h) was found to induce apoptosis, mitochondrial potential (Δψm) depolarization and release of cytochromec in L929-11E cells. Similar responses were found in L929 cells when treated with TNF-α. Heating at 43°C for 1h did not significantly damage the mitochondria of L929-11E cells but partially reversed their resistance to TNF-α. When L929-11E cells were sequentially treated with heating (43°C, 1h) and TNF-α, a more severe damage in mitochondria was observed. Taken together, our results indicate (1) hyperthermia induced apoptosis in L929-11E cells via mitochondrial damages in away very similar to the action of TNF-α in L929 cells, (2) hyperthermia could be used to overcome TNF-α resistance by altering mitochondrial activities and (3) L929-11E and its parental cells provide a useful model in elucidating the signalling linkage between TNF-α receptor and mitochondria. © 2000 Elsevier Science Inc. All rights reserved.
著者Ko S., Yuen W.F., Fung K.P., Lee C.Y., Choy Y.M., Cheng H.K., Kwok T.T., Kong S.K.
期刊名稱Life Sciences
出版年份2000
月份12
日期1
卷號67
期次25
出版社Elsevier BV
出版地Netherlands
頁次3113 - 3121
國際標準期刊號0024-3205
語言英式英語
關鍵詞Hyperthermia, Mitochondria, Mitochondrial potential, Resistance, TNF-α

上次更新時間 2020-11-08 於 00:58