Reversal of TNF-α resistance by hyperthermia: Role of mitochondria
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AbstractThe aim of this study is to examine the effect of hyperthermia on tumour necrosis factor-α (TNF-α) resistance in L929-11E cells. L929-11E is a TNF-α resistant variant derived from L929 cells, a commonly used model for TNF-α study. Based on the results from flow cytometry and Western blotting, hyperthermia (43°C, 3h) was found to induce apoptosis, mitochondrial potential (Δψm) depolarization and release of cytochromec in L929-11E cells. Similar responses were found in L929 cells when treated with TNF-α. Heating at 43°C for 1h did not significantly damage the mitochondria of L929-11E cells but partially reversed their resistance to TNF-α. When L929-11E cells were sequentially treated with heating (43°C, 1h) and TNF-α, a more severe damage in mitochondria was observed. Taken together, our results indicate (1) hyperthermia induced apoptosis in L929-11E cells via mitochondrial damages in away very similar to the action of TNF-α in L929 cells, (2) hyperthermia could be used to overcome TNF-α resistance by altering mitochondrial activities and (3) L929-11E and its parental cells provide a useful model in elucidating the signalling linkage between TNF-α receptor and mitochondria. © 2000 Elsevier Science Inc. All rights reserved.
All Author(s) ListKo S., Yuen W.F., Fung K.P., Lee C.Y., Choy Y.M., Cheng H.K., Kwok T.T., Kong S.K.
Journal nameLife Sciences
Volume Number67
Issue Number25
PublisherElsevier BV
Place of PublicationNetherlands
Pages3113 - 3121
LanguagesEnglish-United Kingdom
KeywordsHyperthermia, Mitochondria, Mitochondrial potential, Resistance, TNF-α

Last updated on 2020-02-07 at 01:22