Transcriptomic alterations in Daphnia magna embryos from mothers exposed to hypoxia
Publication in refereed journal

香港中文大學研究人員
替代計量分析
.

其它資訊
摘要Hypoxia occurs when dissolved oxygen (DO) falls below 2.8 mg L−1 in aquatic environments. It can cause trans-generational effects not only in fish, but also in the water fleas Daphnia. In this study, transcriptome sequencing analysis was employed to identify transcriptomic alterations induced by hypoxia in embryos of Daphnia magna, with an aim to investigate the mechanism underlying the trans-generational effects caused by hypoxia in Daphnia. The embryos (F1) were collected from adults (F0) that were previously exposed to hypoxia (or normoxia) for their whole life. De novo transcriptome assembly identified 18270 transcripts that were matched to the UniProtKB/Swiss-Prot database and resulted in 7419 genes. Comparative transcriptome analysis showed 124 differentially expressed genes, including 70 up- and 54 down-regulated genes under hypoxia. Gene ontology analysis further highlighted three clusters of genes which revealed acclimatory changes of haemoglobin, suppression in vitellogenin gene family and histone modifications. Specifically, the expressions of histone H2B, H3, H4 and histone deacetylase 4 (HDAC4) were deregulated. This study suggested that trans-generational effects of hypoxia on Daphnia may be mediated through epigenetic regulations of histone modifications.
著者Lai K.-P., Li J.-W., Chan C.Y.S., Chan T.-F., Yuen K.W.Y., Chiu J.M.Y.
期刊名稱Aquatic Toxicology
出版年份2016
月份8
日期1
卷號177
出版社Elsevier BV
出版地Netherlands
頁次454 - 463
國際標準期刊號0166-445X
語言英式英語
關鍵詞Embryonic development, Haemoglobin, Histone, Trans-generational effect, Vitellogenin

上次更新時間 2020-16-09 於 02:39