mTHPC-photodynamic therapy induced apoptosis in nasopharyngeal carcinoma cells
Refereed conference paper presented and published in conference proceedings

香港中文大學研究人員
替代計量分析
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其它資訊
摘要In this study, the early apoptotic events of mTHPC-medicated photodynamic therapy (PDT) was explored in two human nasopharyngeal carcinoma (NPC) cell lines - NPC/HK1 cells and NPC/CNE2 cells. Cells (5 × 103) were incubated with mTHPC (0.8 μg/ml) in chamber slides for 20 h and subjected to light irradiation at 2 J/cm2 (LD80). Morphologic changes of treated cells were examined at 0- 4 h after the light irradiation by a light microscopy. The early stage of apoptosis was detected by fluorescein-conjugated Annexin V (Annexin V-FITC) assay. Mitochondrial membrane damage and cytochrome c release were determined by flowcytometric analysis. The Bcl-2 expression was measured by Western blot analysis. One hour after mTHPC-mediated PDT, membrane blebbing and cell shrinkage appeared in both HK1 and CNE2 cells. Annexin V-FITC assay showed that a considerable number of HK1 and CNE2 cells became apoptotic at 1 h after PDT. Flowcytometric analysis showed that the cytochrome c was released at 1 h after PDT. The Bcl-2 expression also declined significantly in both cell lines compared to the control groups. mTHPC-mediated PDT can effectively induce apoptotic responses in NPC cells which might be modulated by mitochondrial damage and Bcl-2 inhibition.
著者Yow C.M.N., Leung A.W.N., Huang Z.
會議名稱Optics in Health Care and Biomedical Optics III
會議開始日12.11.2007
會議完結日15.11.2007
會議地點Beijing
會議國家/地區中國
出版年份2008
月份5
日期15
卷號6826
國際標準書號9780819470010
國際標準期刊號1605-7422
語言英式英語
關鍵詞Apoptosis, mTHPC, Nasopharyngeal carcinoma, Photodynamic therapy PDT

上次更新時間 2020-25-10 於 02:36