Interrogation of genomes by molecular copy-number counting (MCC)
Publication in refereed journal

香港中文大學研究人員
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摘要Human cancers and some congenital traits are characterized by cytogenetic aberrations including translocations, amplifications, duplications or deletions that can involve gain or loss of genetic material. We have developed a simple method to precisely delineate such regions with known or cryptic genomic alterations. Molecular copy-number counting (MCC) uses PCR to interrogate miniscule amounts of genomic DNA and allows progressive delineation of DNA content to within a few hundred base pairs of a genomic alteration. As an example, we have located the junctions of a recurrent nonreciprocal translocation between chromosomes 3 and 5 in human renal cell carcinoma, facilitating cloning of the breakpoint without recourse to genomic libraries. The analysis also revealed additional cryptic chromosomal changes close to the translocation junction. MCC is a fast and flexible method for characterizing a wide range of chromosomal aberrations. © 2006 Nature Publishing Group.
著者Daser A., Thangavelu M., Pannell R., Forster A., Sparrow L., Chung G., Dear P.H., Rabbitts T.H.
期刊名稱Nature Methods
出版年份2006
月份6
日期1
卷號3
期次6
出版社Nature Publishing Group
出版地United Kingdom
頁次447 - 453
國際標準期刊號1548-7091
電子國際標準期刊號1548-7105
語言英式英語

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