Dendrobium officinale extract increases the expression of aquaporin 5 through activation of ERK and JNK in lacrimal gland cells
Refereed conference paper presented and published in conference proceedings


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AbstractDendrobium officinale has been used as a healthy food and a source of nutrients for thousands of years in China. It is traditionally used to nourish stomach, maintain body fluid production and treat thirst, sore throat and blurred vision. Dendrobium officinale and its formulations are used to promote body fluid production in dry eye disease or hyposalivation of patients after head and neck radiotherapy. Recently, polysaccharides isolated from Dendrobium officinale have been demonstrated to protect salivary gland and stimulate expression of aquaporin 5 (AQP5) in labial glands from Sjögren’s syndrome patients. However, the underlying mechanism of promotive effect of Dendrobium officinale on body fluid secretion is seldom reported. Therefore, our study aimed to determine whether Dendrobium officinale aqueous extract could increase the expression of AQP5 through activation of MAPKs pathways in primary cultured lacrimal gland cell. Primary lacrimal gland cells were isolated from male SD rats and characterized by immunofluorescence. Cell viability and cell proliferation were evaluated by MTT assay and BrdU assay, respectively. The results showed aqueous extract of Dendrobium officinale (DOW) increased the lacrimal gland cell viability, but not in cell proliferation. The mRNA levels of β-hexosaminidase A (HexA) and AQP5 were increased after DOW treatment for 4 hours by RT-PCR. Protein levels were measured by western blotting. The increase of AQP5 expression was greater after 24-hour and returned to its ordinary level by 48-hour treatment at dose of 800 μg/ml. Moreover, DOW stimulated phosphorylation of ERK and JNK with 800 μg/ml treatment and peaked after 30 min stimulation. In conclusion, we firstly presented evidence that Dendrobium officinale aqueous extract increased the expression of aquaporin 5 through activation of ERK and JNK pathways in lacrimal gland cells. Our data has provided a solid background for Dendrobium officinale to be developed as an alternative or adjuvant intervention in dry eye disease.
All Author(s) ListZeng Q, Ko CH, Siu WS, Wong CW, Hu JM, Lau CBS, Leung PC
Name of ConferenceThe Experimental Biology 2017
Start Date of Conference22/04/2017
End Date of Conference26/04/2017
Place of ConferenceChicago
Country/Region of ConferenceUnited States of America
Proceedings TitleThe FASEB Journal
Year2017
Month4
Volume Number31
Issue Number1 Supplement
LanguagesEnglish-United Kingdom

Last updated on 2018-22-01 at 09:17