Bioinformatics for Novel Long Intergenic Noncoding RNA (lincRNA) Identification in Skeletal Muscle Cells
Chapter in an edited book (author)

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AbstractLong intergenic noncoding RNAs (lincRNAs) have emerged as critical participators in gene regulation in myriads of cell types. The development of the whole transcriptome sequencing technology, or RNA-seq , has enabled novel lincRNA detection, but the bioinformatics analysis toward distinguishing reliable ones remains a challenge. Here, we describe the bioinformatics workflow developed for identifying novel lincRNAs step by step, including read alignment, transcriptome assembly and transcript filtering.
All Author(s) ListXianlu Peng, Kun Sun, Jiajian Zhou, Hao Sun, Huating Wang
All Editor(s) ListEusebio Perdiguero, DDW Cornelison
Journal nameMethods in molecular biology (Clifton, N.J.)
Book titleMuscle Stem Cells
Series TitleMethods in Molecular Biology
Volume Number1556
PublisherSpringer New York
Pages355 - 362
LanguagesEnglish-United Kingdom
KeywordslincRNA, RNA-seq, Bioinformatics, Skeletal muscle cells

Last updated on 2020-06-07 at 00:00