Differential Expression of LBX1 in paravertebral muscles and vertebra of Chinese patients with adolescent idiopathic scoliosis: a preliminary study
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摘要Introduction: Adolescent idiopathic scoliosis (AIS) is the most common pediatric spinal deformity, affecting 1% to 3% of adolescents worldwide. However, the true etiology of idiopathic scoliosis remains unknown and is considered to be multifactorial. In recent genome-wide association studies by us and our collaborators, we have demonstrated a strong association between AIS and SNPs near the ladybird homeobox 1 (LBX1) locus. Despite the expression of LBX1 being reported in skeletal muscles, the expression in bone tissues and its association with idiopathic scoliosis remains unknown. In this study, we aim to investigate LBX1 mRNA expression in bilateral paravertebral muscles and apical vertebra in AIS and control subjects and its association with the development of scoliosis.

Subjects and Methods: Sixteen AIS patients and 3 non-AIS controls were included. Muscle samples were harvested from bilateral paravertebral muscles at the apical vertebral level. LBX1 mRNA expression was evaluated by the conventional real-time PCR. LBX1 expressions in bilateral paravertebral muscles were compared in each group. The expression ratio, the expression at the convex side relative to the concave side, was compared between groups.In a separate cohort, apical vertebra biopsies were retrieved from 9 AIS patients and 3 non-AIS control. LBX1 mRNA expression was evaluated by the conventional real-time PCR. LBX1 expressions in the apical vertebra were compared in each group. The expression ratio, the expression between osteopenic and nonosteopenic in AIS, was compared between groups.

Results: In paravertebral muscle, the LBX1 expression in AIS group was higher than the controls. When we further subgroup the samples to convex and concave sides between the AIS, there was a significantly higher LBX1 mRNA expression at the convex side (p<0.05). While on evaluating the expression ratio of LBX1 in apical vertebra, the AIS group have a lowered expression than controls (p<0.01). When furthered subgroup on the patient being clinically-evaluated as osteopenic, AIS patients with osteopenia (n=3) has a significantly lower expression of LBX1 than non-osteopenic AIS patients (n=6) (p<0.01).

Discussion and Conclusion: There was a difference on the LBX1 mRNA expression between AIS group and controls. In the AIS group, the expression was asymmetrically observed in the apical paraspinal muscles. This is the first study to show the lower mRNA LBX1 expression in the apical vertebra in the AIS than controls. In addition, there was a significantly lower LBX1 expression on the apical vertebra in AIS patients with osteopenia. These findings suggest the possible cross-talk between muscle and bone homeostasis and their possible contribution to development or progression of the spinal deformity in AIS.
著者G. C. W. Man, N. L. S. Tang, W. Y. W. Lee, J. Zhang, B. K. W. Ng, L. L. Xu, Y. Qiu, J. C. Y. Cheng
會議名稱2016 International Combined Meeting of Orthopaedics Research Societies
會議開始日21.09.2016
會議完結日25.09.2016
會議地點Xi'an, China
會議國家/地區中國
出版年份2016
語言英式英語

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