Identification of Genes Associated with Pancreatic Cancer Metastasis by Genome-wide CRISPR Cas9 Screening
Refereed conference paper presented and published in conference proceedings


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AbstractPancreatic cancer is an extremely lethal cancer with a 5-year survival rate of 7%. One major reason why pancreatic cancer is hard to treat is its metastasis nature. Genes that related to the tumorigenesis and progression of pancreatic cancer were identified after decades of efforts. Several researches had revealed that pancreatic cancer originated from the successive accumulation of gene mutations, especially KRAS2, CNKD2A, TP53 and SMAD4. However, little is known about genes that regulate pancreatic cancer metastasis. Here we used a genome wide CRISPR Cas9 screening to identify the gene(s) associated with the metastasis of pancreatic cancer. We transduced pancreatic cancer cell line with lentivirus-based genome wide CRISPR sgRNA library to establish mutated cell library, and orthotopically injected the cell library into nude mice and waited for primary tumor growth and metastasis, then collected primary and metastatic tumors after several months. By next generation sequencing and analysis, we identified some gene candidates which may play important roles on pancreatic cancer metastasis.
All Author(s) ListLi YJ, Feng HY, Liu GJ, Wong CH, Li CH, Chen YC
Name of ConferenceAmerican Association for Cancer Research Annual Meeting 2017
Start Date of Conference01/04/2017
End Date of Conference05/05/2017
Place of ConferenceWashington
Country/Region of ConferenceUnited States of America
Year2017
Month4
LanguagesEnglish-United States

Last updated on 2018-22-01 at 09:14