Is It Possible To Identify Constituent Herbs In Decoctions By Molecular Approach?
Refereed conference paper presented and published in conference proceedings

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AbstractGenomic DNA provides a rich source of material for species identification [1]. However, degradation of DNA during the processing procedures makes people believe that molecular authentication is unsuitable for herbal decoctions, a common form of herbal products. Using ginseng decoctions prepared by boiling for 30, 60 and 120 min as examples, we show that DNA may be extracted from these preparations for polymerase chain reaction (PCR) and DNA sequencing. We also found: (a) sample pulverization before decoction boiling improved PCR detection, (b) prolonged boiling increased the DNA concentration but decreased the intactness of DNA. At 120 min, only DNA with size of less than 191 bp was detected. We have further shown that molecular authentication could be performed on a decoction consisting of four herbs and a commercial ready-to-serve soup [2]. We are investigating if molecular techniques can be used to quantify herbal components in decoctions. Our study has enhanced the value of molecular techniques in the quality control of herbal material.

Acknowledgement: Thanks are due to Mr. Ka-Lok Wong and Dr. Li-Li Jiang for helpful discussion and Dr. David Tai-Wai Lau, Curator of the Shiu-Ying Hu herbarium, School of Life Sciences, The Chinese University of Hong Kong for authenticating the herbs by organoleptic characteristics.

References: [1] Zhang YB, Shaw PC, et al. (2007)J Food Drug Anal, 15: 1 – 9. [2] Lo YT, Li M, et al. (2015) Chin Med, 10 (1):1.
All Author(s) ListLo Y, Li M, Shaw P
Name of ConferenceThe Joint Meeting of 16th Annual International Conference on the Science of Botanicals (ICSB) with 5th Interim American Society of Pharmacognosy (ASP)
Start Date of Conference11/04/2016
End Date of Conference14/04/2016
Place of ConferenceOxford, Mississippi
Country/Region of ConferenceUnited States of America
Journal namePlanta Medica
Proceedings TitlePlanta Medica
Year2016
Volume Number82
Issue Number05
PagesPC43 - PC43
ISSN0032-0943
eISSN1439-0221
LanguagesEnglish-United Kingdom

Last updated on 2020-05-07 at 23:59