Confirmed presence of bacterial 16S rRNA in Achilles' tendon rupture samples
Refereed conference paper presented and published in conference proceedings

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AbstractIntroduction: Tendinopathy continues to be a growing concern in the orthopaedic arena, with various theories about its aetiopathogenesis and, given the insidious nature of the disease, it is this information about the early stages of the disease that we lack in order to make significant advancement in the treatment of this disease. Myocarditis is often caused by viruses and bacteria, and it is this microbial involvement that causes degradation of the supportive collagen matrix [1], as well as the activation of nucleotide-binding oligomerization domain (NOD) proteins that can cause cardiac dysfunction, fibrosis and apoptosis [2], similar to features observed in tendinopathy. We previously demonstrated [3] that NOD1 was significantly more prevalent in tendinopathy samples than in healthy samples, as well as demonstrating that in vitro tendon-derived stem cells responded to diaminopimelic acid (DAP; a bacterial NOD antagonist) with a pro-inflammatory response. There have been at least two reported cases of tendinopathy associated with Borrelia burgdorferi (Lyme disease) [4,5] and one reported case caused by Mycobacterium tuberculosis [6]. We aim to detect the presence of 16S rRNA, a highly conserved gene-coding region in bacteria in human tendinopathy samples.
Methods: In an ongoing study, 24 Achilles’ tendon rupture samples were collected that displayed evidence of tendinopathic changes (confirmed by MRI) and 24 healthy hamstring samples were collected from ACL reconstruction grafts under sterile conditions (Approved by the Clinical Research Ethics Committee of the authors’ institution; Ref no.: CRE-2013.479). Genomic DNA was extracted from all samples and universal 16S primers (27F and 1492R; Invivogen©) were used to conduct PCR in order to confirm the presence or absence of bacterial 16S rRNA (confirmed by agarose gel electrophoresis). Escherichia coli was used as a positive control, while a blank reagent was used as a negative control. The Fisher’s exact test was used to determine whether there were significant differences between the number of 16S rRNA positive cases in the tendinopathy group and the healthy tendon group, as well as the female: male gender distribution differences between the tendinopathy and healthy groups. A t-test was used to determine whether there was a significant difference in ages between the tendinopathy group and the healthy tendon group. p-value<0.05 was considered significant.
All Author(s) ListChelsea Hopkins, Sai-Chuen Fu, Kai-Ming Chan, Goran Friman, Ling Qin, Christer G Rolf
Name of Conference2016 Congress of Asia-Pacific Knee, Arthroscopy and Sports Medicine Society (APKASS), and the International Forum of Orthopaedics Sports Medicine and Arthroscopic Surgery (IFOSMA)
Start Date of Conference09/06/2016
End Date of Conference12/06/2016
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Proceedings TitleAsia-Pacific Journal of Sports Medicine, Arthroscopy, Rehabilitation and Technology
Year2016
Month10
Volume Number6
PublisherElsevier (Singapore) Pte Ltd
Place of PublicationSingapore
Pages20 - 20
LanguagesEnglish-United Kingdom

Last updated on 2020-05-07 at 00:09