Involvement of Melatonin Receptors with Bone Mineralization During Embryonic Skeletal Development
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AbstractIntroduction: Melatonin, a hormone mainly synthesised and secreted by the pineal gland, was found recently to play a
regulatory role on bone physiology. Plasma melatonin level declined progressively in elders in association with osteoporosis. Abnormal melatonin receptor expression was also observed in adolescent idiopathic scoliosis (AIS). This study aimed to determine the little known temporal expression of melatonin receptors during embryonic skeletal development and the corresponding bone mineralisation in an animal model.

Methods: Mice at embryonic day 14.5 (E14.5), 15.5 (E15.5), 16.5 (E16.5), 17.5 (E17.5), 18.5 (E18.5) to postnatal day 0 (P0) were used in this study. Embryos were fixed for micro–computed tomography (Micro-CT) and histological analysis for bone mineralisation. Presence of melatonin receptors (MTNR1a and MTNR1b) was detected by immunofluorescent staining. In addition, whole skeleton was isolated for mRNA quantification of receptor expressions.

Results: Histological and micro-CT analysis revealed the progressively increased formation of bony tissues, bone mineral density, and bone volume / total volume from E14.5 to P0. The increase was found to match the quantitative increase of MTNR1a receptor in ossified regions of the skeleton across different gestational age. However, minimal changes of MTNR1b were detected.

Conclusion: The new findings on the temporal physiological changes of MTNR1a receptors and its link to the ossification pattern, bone formation, and bone quality could provide important information that might have aetiopathogenetic and potential therapeutic implications in conditions like AIS and other bone metabolic abnormalities.
Acceptance Date05/11/2016
All Author(s) ListG. C. W. Man, N. L. S. Tang, B. K. W. Ng, Y. Qiu, J. C. Y. Cheng
Name of ConferenceThe 36th Annual Congress of the Hong Kong Orthopaedic Association (HKOA)
Start Date of Conference05/11/2016
End Date of Conference06/11/2016
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Year2016
LanguagesEnglish-United Kingdom

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