Screening and analysis of potential anti-tumor components from the stipe of Ganoderma sinense using high-performance liquid chromatography/time-of-flight mass spectrometry with multivariate statistical tool
Publication in refereed journal


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替代計量分析
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摘要According to Chinese Pharmacopoeia 2015 edition, Ganoderma (Lingzhi) is a species complex that comprise of Ganoderma lucidum and Ganoderma sinense. The bioactivity and chemical composition of G. lucidium had been studied extensively, and it was shown to possess antitumor activities in pharmacological studies. In contrast, G. sinense has not been studied in great detail. Our previous studies found that the stipe of G. sinense exhibited more potent antitumor activity than the pileus. To identify the antitumor compounds in the stipe of G. sinense, we studied its chemical components by merging the bioactivity results with liquid chromatography-mass spectrometry-based chemometrics.The stipe of G. sinense was extracted with water, followed by ethanol precipitation and liquid-liquid partition. The resulting residue was fractionated using column chromatography. The antitumor activity of these fractions were analysed using MTT assay in murine breast tumor 4T1 cells, and their chemical components were studied using the LC-QTOF-MS with multivariate statistical tools. The chemometric and MS/MS analysis correlated bioactivity with five known cytotoxic compounds, 4-hyroxyphenylacetate, 9oxo-(10E,12E)-octadecadienoic acid, 3-phenyl-2-propenoic acid, 13-oxo-(9E,11E)-octadecadienoic acid and lingzhine C, from the stipe of G. sinense. To the best of our knowledge, 4-hyroxyphenylacetate, 3-phenyl-2-propenoic acid and lingzhine C are firstly reported to be found in G. sinense. These five compounds will be investigated for their antitumor activities in the future.
著者Chan KM, Yue GGL, Li P, Wong ECW, Lee JKM, Kennelly EJ, Lau CBS
期刊名稱Journal of Chromatography A
出版年份2017
月份3
日期3
卷號1487
出版社ELSEVIER SCIENCE BV
頁次162 - 167
國際標準期刊號0021-9673
電子國際標準期刊號1873-3778
語言英式英語
關鍵詞Ganoderma sinense, Chemometrics, Anti-tumor, LC-QTOF-MS, Lingzhine C, 4-hyroxyphenylacetate, 3-phenyl-2-propenoic acid
Web of Science 學科類別Biochemical Research Methods;Chemistry, Analytical;Biochemistry & Molecular Biology;Chemistry

上次更新時間 2020-11-10 於 00:38