α2-COP is involved in early secretory traffic in Arabidopsis and is required for plant growth.
Publication in refereed journal
Full Text
Digital Object Identifier (DOI) View Full-text in Publisher Website |
Altmetrics Information
.
Other information
AbstractCOP (coat protein) I-coated vesicles mediate intra-Golgi transport and retrograde transport from the Golgi to the endoplasmic reticulum. These vesicles form through the action of the small GTPase ADP-ribosylation factor 1 (ARF1) and the COPI heptameric protein complex (coatomer), which consists of seven subunits (α-, β-, β'-, γ-, δ-, ε- and ζ-COP). In contrast to mammals and yeast, several isoforms for coatomer subunits, with the exception of γ and δ, have been identified in Arabidopsis. To understand the role of COPI proteins in plant biology, we have identified and characterized a loss-of-function mutant of α2-COP, an Arabidopsis α-COP isoform. The α2-cop mutant displayed defects in plant growth, including small rosettes, stems and roots and mislocalization of p24δ5, a protein of the p24 family containing a C-terminal dilysine motif involved in COPI binding. The α2-cop mutant also exhibited abnormal morphology of the Golgi apparatus. Global expression analysis of the α2-cop mutant revealed altered expression of plant cell wall-associated genes. In addition, a strong upregulation of SEC31A, which encodes a subunit of the COPII coat, was observed in the α2-cop mutant; this also occurs in a mutant of a gene upstream of COPI assembly, GNL1, which encodes an ARF-guanine nucleotide exchange factor (GEF). These findings suggest that loss of α2-COP affects the expression of secretory pathway genes.
All Author(s) ListGimeno-Ferrer, Pastor-Cantizano, Bernat-Silvestre, Selvi-Martínez, Vera-Sirera, Gao, Perez-Amador, Jiang, Aniento, Marcote
Journal nameJournal of experimental botany
Year2017
Volume Number68
Issue Number3
Pages391 - 401
ISSN0022-0957
LanguagesEnglish-United Kingdom
Keywordsα1-COP, α2-COP, Arabidopsis, coat protein, COPI, COPII, Golgi apparatus, p24 family protein, SEC31
