Puerarin offsets the anticoagulation effect of warfarin in rats by inducing rCyps, upregulating vitamin K epoxide reductase and inhibiting thrombomodulin
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AbstractPurposeThe current study was conducted to investigate the potential pharmacokinetic and pharmacodynamic interactions between warfarin and puerarin which is the most abundant component in Pueraria lobata (Gegen). In vivo and ex vivo rat models were used to reveal the underlying mechanisms of such interactions.MethodsApart from one control group, five groups of Sprague-Dawley rats were treated with warfarin, oral puerarin, oral puerarin with warfarin, intravenous puerarin, intravenous puerarin with warfarin. The treatment lasted for 5 consecutive days. Thereafter, the levels of warfarin, warfarin metabolites and puerarin in plasma of these rats were monitored and compared. The rCyps activity and expression in rat livers of different treatment groups were assessed. The prothrombin time was observed. The vitamin K epoxide reductase (VKOR) activity and expression in rat livers were evaluated. Thrombomodulin activity and expression in the rat lung and rat plasma were assessed. The soluble thrombomodulin (sTM) concentrations of different treatment groups were examined.ResultsIntravenously administered puerarin altered the pharmacokinetics of warfarin significantly by shortening t(1/2), decreasing AUC(0-96 h) and increasing the clearance of warfarin. Further mechanistic studies suggested that both oral and intravenous administration of puerarin significantly induced the activities and expressions of rCyp2b1, rCyp2c6 and rCyp1a1. In addition, co-administration of puerarin reduced the prothrombin time of rat plasma by enhancing VKOR and inhibiting thrombomodulin.ConclusionPuerarin increased warfarin metabolism and offset warfarin anticoagulation by inducing rCyps, upregulating VKOR and inhibiting thrombomodulin in rats. The clinical impact of such potential interactions warrants further verification.
All Author(s) ListGe BK, Zhang Z, Lam TT, Zuo Z
Journal nameBiopharmaceutics and Drug Disposition
Year2017
Month1
Volume Number38
Issue Number1
PublisherWILEY-BLACKWELL
Pages33 - 49
ISSN0142-2782
eISSN1099-081X
LanguagesEnglish-United Kingdom
Keywordswarfarin, puerarin, liver cytochrome P450, vitamin K epoxide reductase, thrombomodulin
Web of Science Subject CategoriesPharmacology & Pharmacy;Pharmacology & Pharmacy

Last updated on 2021-03-04 at 01:06