MicroRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer
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AbstractObjective: Detection of microRNA (miRNA) aberrations in human faeces is a new approach for colorectal cancer (CRC) screening. The aim of this study was to characterise miR-20a in faeces as a non-invasive biomarker for diagnosis of CRC. Design: miR-20a was selected from an expression microarray containing 667 miRNAs. Further verification of miR-20a was performed in 40 pairs of primary CRC tissues, as well as 595 faecal samples (198 CRCs, 199 adenomas, and 198 healthy controls) using TaqMan probe based quantitative Real-Time PCR (qRT-PCR). Results: miR-20a expression was significantly higher in the 40 CRC tumours compared to their respective adjacent normal tissues (P = 0.0065). Levels of miR-20a were also significantly higher in faecal samples from CRC patients (P < 0.0001). The area under receiver operating characteristic (AUROC) curve for miR-20a was 0.73, with a sensitivity of 55% and specificity of 82% for CRC patients compared with controls. No significant difference in the level of miR-20a was found between patients with proximal, distal, and rectal cancer. The use of antibiotics did not influence faecal miR-20a levels. Conclusions: Faecal-based miR-20a can be utilised as a potential non-invasive biomarker for CRC screening.
All Author(s) ListYau T.O., Wu C.W., Tang C.-M., Chen Y., Fang J., Dong Y., Liang Q., Man Ng S.S., Leung Chan F.K., Yiu Sung J.J., Yu J.
Journal nameOncotarget
Volume Number7
Issue Number2
PublisherImpact Journals
Place of PublicationUnited States
Pages1559 - 1568
LanguagesEnglish-United Kingdom
KeywordsColorectal cancer, Diagnosis, MicroRNA, Non-invasive, Stool biomarker

Last updated on 2020-29-10 at 00:34