An efficient way of studying protein-protein interactions involving HIF-α, c-Myc, and Sp1
Chapter in an edited book (author)

Times Cited
Altmetrics Information

Other information
AbstractProtein-protein interaction is an essential biochemical event that mediates various cellular processes including gene expression, intracellular signaling, and intercellular interaction. Understanding such interaction is key to the elucidation of mechanisms of cellular processes in biology and diseases. The hypoxia-inducible transcription factor HIF-1α possesses a non-transcriptional activity that competes with c-Myc for Sp1 binding, whereas its isoform HIF-2α lacks Sp1-binding activity due to phosphorylation. Here, we describe the use of in vitro translation to effectively investigate the dynamics of protein-protein interactions among HIF-1α, c-Myc, and Sp1 and to demonstrate protein phosphorylation as a molecular determinant that functionally distinguishes HIF-2α from HIF-1α. © 2013 Springer Science+Business Media, LLC.
All Author(s) ListTo K.K.-W., Huang L.E.
All Editor(s) ListLaura Soucek, Nicole M. Sodir
Book titleThe Myc Gene: methods and protocols
Series TitleMethods in Molecular Biology
Detailed descriptioned. by Soucek, Laura; Sodir, Nicole M.
Volume Number1012
PublisherHumana Press
Place of PublicationUnited States
Pages77 - 84
LanguagesEnglish-United Kingdom
Keywordsc-Myc, HIF-1α, HIF-2α, In vitro coupled transcription/translation, Protein-protein interaction, Rabbit reticulocyte lysate, Sp1, Wheat germ extract

Last updated on 2021-17-09 at 23:44