Cell-free circulating tumor RNAs in plasma as the potential prognostic biomarkers in colorectal cancer
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AbstractBackground: Cell free RNA (cfRNA) contains transcript fragments from multiple cell types, making it useful for cancer detection in clinical settings. However, the pathophysiological origins of cfRNAs in plasma from colorectal cancer (CRC) patients remain unclear.

Methods: To identify the tissue-specific contributions of cfRNAs transcriptomic profile, we used a published single-cell transcriptomics profile to deconvolute cell type abundance among paired plasma samples from CRC patients who underwent tumor-ablative surgery. We further validated the differentially expressed cfRNAs in 5 pairs of CRC tumor samples and adjacent tissue samples as well as 3 additional CRC tumor samples using RNA-sequencing.

Results: The transcriptomic component from intestinal secretory cells was significantly decreased in the in-house post-surgical cfRNA. The HPGD, PACS1, and TDP2 expression was consistent across cfRNA and tissue samples. Using the Cancer Genome Atlas (TCGA) CRC datasets, we were able to classify the patients into two groups with significantly different survival outcomes.

Conclusions: The three-gene signature holds promise in applying minimal residual disease (MRD) testing, which involves profiling remnants of cancer cells after or during treatment. Biomarkers identified in the present study need to be validated in a larger cohort of samples in order to ascertain their possible use in early diagnosis of CRC.
All Author(s) ListNana Jin, Chau-Ming Kan, Xiao Meng Pei, Wing Lam Cheung, Simon Siu Man Ng, Heong Ting Wong, Hennie Yuk-Lin Cheng, Wing Wa Leung, Yee Ni Wong, Hin Fung Tsang, Amanda Kit Ching Chan, Yin Kwan Evelyn Wong, William Chi Shing Cho, John Kwok Cheung Chan, William Chi Shing Tai, Ting-Fung Chan, Sze Chuen Cesar Wong, Aldrin Kay-Yuen Yim, Allen Chi-Shing Yu
Journal nameFrontiers in Oncology
Volume Number13
Article number1134445
LanguagesEnglish-United States
Keywordscell-free circulating tumor RNAs, colorectal (colon) cancer, CRC prognostic biomarkers, RNA sequencing (RNA-seq), transcriptome (RNA-seq)

Last updated on 2024-09-04 at 00:10