Echinacea purpurea (L.) Moench modulates human T-cell cytokine response
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AbstractThe study objective was to evaluate the composition of a neutral and weakly acidic water-soluble extract from Echinacea purpurea (L.) Moench (EchNWA) previously shown to modify murine influenza infection, and to assess immunomodulatory effects on human T-cells. EchNWA extract from fresh aerial parts was extracted with water, ethanolic precipitation, and size-exclusion chromatography. The chemical profile of EchNWA was characterized by chromatography (size-exclusion, HPLC, GC-MS), and small molecule fingerprint analysis performed by HPLC-PDA. Jurkat T-cells at high and low cell density were pretreated or not with doses of EchNWA, followed by activation with phorbol 12-myristate 13-acetate plus ionomycin (PMA+I). Interleukin-2 (IL-2) and interferon gamma (IFNg) cytokine secretions were measured by multi-cytokine luminex technology. Results showed that EchNWA contains 80% polysaccharides, predominantly a 10 kDa entity; phenolic compounds, cynarin, cichoric and caftaric acids, but no detectable alkylamides. Cytokine production required stimulation and was lower after PMA+I activation in high-density compared to low-density conditions. EchNWA mediated a strong dose-dependent enhancement of high-density T-cell production of IL-2 and IFNg response to PMA+I. EchNWA alone did not stimulate T-cells. EchNWA enhanced mean fluorescence intensity of IL-2 in Jurkat T-cells activated by PMA+1 or ionomycin alone. Conversely EchNWA mediated modest but significant suppression of IFNg response and reduced the percentage of CD25+ T-cells under low-density conditions. Conclusions are that EchNWA polysaccharides, but not phenolic compounds have dose-related adjuvant effects on human T-cell cytokine responses characterized by enhancing and suppressive effects that are regulated by T-cell density. © 2014 Elsevier B.V.
All Author(s) ListFonseca F.N., Papanicolaou G., Lin H., Lau C.B.S., Kennelly E.J., Cassileth B.R., Cunningham-Rundles S.
Journal nameInternational Immunopharmacology
Volume Number19
Issue Number1
PublisherElsevier BV
Place of PublicationNetherlands
Pages94 - 102
LanguagesEnglish-United Kingdom
KeywordsCytokine response, Echinacea, Human T-cell density, Immune modulation, Neutral and weakly acidic water-soluble extract, Polysaccharides and phenolic compounds, Small molecule fingerprint-analysis

Last updated on 2020-04-06 at 23:38