Intrarectal administration of mCRAMP-encoding plasmid reverses exacerbated colitis in Cnlp-/- mice
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AbstractCathelicidin is a pleiotropic host defense peptide secreted by epithelial and immune cells. Whether endogenous cathelicidin is protective against ulcerative colitis, however, is unclear. Here we sought to delineate the role of endogenous murine cathelicidin (mCRAMP) and the therapeutic efficacy of intrarectal administration of mCRAMP-encoding plasmid in ulcerative colitis using dextran sulfate sodium (DSS)-challenged cathelicidin-knockout (Cnlp -/-) mice as a model. Cnlp-/- mice had more severe symptoms and mucosal disruption than the wild-type mice in response to DSS challenge. The tissue levels of interleukin-1β and tumor necrosis factor-α, myeloperoxidase activity and the number of apoptotic cells were increased in the colon of DSS-challenged Cnlp-/- mice. Moreover, mucus secretion and mucin gene expression were impaired in Cnlp-/- mice. All these abnormalities were reversed by the intrarectal administration of mCRAMP or mCRAMP-encoding plasmid. Taken together, endogenous cathelicidin may protect against ulcerative colitis through modulation of inflammation and mucus secretion. © 2013 Macmillan Publishers Limited All rights reserved.
All Author(s) ListTai E.K.K., Wu W.K.K., Wang X.J., Wong H.P.S., Yu L., Li Z.J., Lee C.W., Wong C.C.M., Yu J., Sung J.J.Y., Gallo R.L., Cho C.H.
Journal nameGene Therapy
Detailed descriptionTo ORKTS: Updated this record and deleted duplication (P133544) on 24-Feb-2015.
Volume Number20
Issue Number2
PublisherNature Publishing Group
Place of PublicationUnited Kingdom
Pages187 - 193
LanguagesEnglish-United Kingdom
KeywordsAntimicrobial peptide, Cathelicidin, IL-1, Mucin, TNF, Ulcerative colitis

Last updated on 2021-19-09 at 23:55