A Drug Delivery System For 'musculoskeleton Strengthening Herbs' For Approaching Bone Formation Surfaces
Refereed conference paper presented and published in conference proceedings


摘要Objective(s): 'Musculoskeleton strengthening herbals' can't exert obvious beneficial effects on muscle and bone in skeletal disorder therapy mainly due to short retention time in blood and insufficient concentration in bone to execute its bone anabolic effect, so our present study aim to develop nanoparticles entrapping 'musculoskeleton strengthening herbs' approaching bone formation surfaces to enhance the interaction of herbs with osteogenic cells.
Material & Methods: (AspSerSer)6 as targeting moiety for approaching bone formation surfaces was conjugated to the surface of nanoparticles composed of poly(ethyleneglycol)- poly(D,L-lactic-co-glycolic acid) (PEG-PLGA). The Fructus Psoraleae extract and the mixture of Epimedium and Bacopin extract, which are commercially available injection solution, were used as model drugs, respectively. The physical properties were characterized and in vitro drug release was also investigated. The proliferation and osteogenic differentiation of Fructus Psoraleae extract and the mixture of Epimedium and Bacopin extract entrapped in (AspSerSer)6-PEG-PLGA nanoparticles were determined. In in vivo study, (AspSerSer)6-PEG-PLGA nanoparticles carrying DiR as tracer were employed to examine distribution at organ, tissue and cell levels in rats, respectively.
Results: The average particle size of (AspSerSer)6-PEGPLGA nanoparticles entrapping the herbal extracts was about 118±5 nm. It had slow release ability and significantly promoted osteoblast differentiation and osteogenesis in vitro compared to Fructus Psoraleae extract and the mixture of Epimedium and Bacopin extract without delivery systems, respectively. In in vivo study, the signals in femora from rats injected with (AspSerSer)6-PEG-PLGA nanoparticles showed more intensive than that injected with PEG-PLGA nanoparticles without (AspSerSer)6 group at 4 h after administration. Moreover, fluorescence signals remained intense in femora after administration of (AspSerSer)6-PEGPLGA nanoparticles at 24 h. We found that bone formation surfaces labeled with calcein green were largely colocalized with rhodamine labeled (AspSerSer)6-PEG-PLGA and also found numerous instances of co-localization of DiR with ALP- or RUNX2- positive cells when (AspSerSer)6-PEGPLGA nanoparticles as delivery system, whereas there were few instances of such overlapping staining when PEGPLGA nanoparticles as carriers.
Conclusion(s): (AspSerSer)6-PEG-PLGA nanoparticles not only maintained drugs slow release, enhanced bioactivities, but also facilitated 'Musculoskeleton strengthening herbals' approaching bone formation surfaces to exerting anabolic effects.
著者Heng Wu, Baosheng Guo, Baoting Zhang, Chao Liang, Zhijun Yang, Zhibo Hou, Ling Qin, Ge Zhang
會議名稱IOF-ECCEO European Congress on Osteoporosis and Osteoarthritis / 2nd IOF-ESCEO Pre-Clinical Symposium
期刊名稱Osteoporosis International
頁次S293 - S293
Web of Science 學科類別Endocrinology & Metabolism;Endocrinology & Metabolism;ENDOCRINOLOGY & METABOLISM

上次更新時間 2022-15-01 於 00:54