Neuronal Differentiation of C17.2 Neural Stem Cells Induced by a Natural Flavonoid, Baicalin
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AbstractNatural medicinal materials are a significant resource for the identification of compounds with specific biological properties. In this study, we employed multipotent C17.2 neural stem cells as a model for screening natural compounds that possess neural differentiation-inducing properties. We show here for the first time that, out of the 67 compounds tested, the flavonoid baicalin is a potent differentiation-inducing agent. Baicalin increased the number of cells bearing extended neurites and the expression levels of a number of neuronal markers. Importantly, baicalin promoted the expression of several key neurogenic transcriptional factors. Moreover, we demonstrated that baicalin enhanced the phosphorylation/activation of Erk1/2. Inhibition of Erk1/2 activation by the MEK inhibitor U0126 attenuated the neuronal differentiation-inducing effect of baicalin. Taken together, our findings suggest that baicalin induces neuronal differentiation of C17.2 neural stem cells and that this is mediated by activation of Erk1/2. Our work lays the foundation for exploring baicalin for the promotion of neural regeneration after injury or disease. Neuroregeneration: Baicalin was identified as a potent differentiation-inducing compound in a screening platform based on C17.2 neural stem cells. This natural flavonoid significantly increases Erk1/2-activation-mediated neuronal differentiation. Our findings provide a scientific basis for the therapeutic potential of baicalin for neuroregeneration. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
All Author(s) ListLi M., Tsang K.-S., Choi S.-T., Li K., Shaw P.-C., Lau K.-F.
Journal nameChemBioChem
Volume Number12
Issue Number3
PublisherJohn Wiley & Sons Ltd.
Place of PublicationUnited Kingdom
Pages449 - 456
LanguagesEnglish-United Kingdom
KeywordsBaicalin, Biological activity, Erk1/2, Natural products, Neurological agents

Last updated on 2021-12-10 at 23:58