Whole extracts of Radix Achyranthis Bidentatae and Radix Cyathulae promote angiogenesis in human umbilical vein endothelial cells in vitro and in zebrafish in vivo
Publication in refereed journal

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其它資訊
摘要Although Radix Achyranthis Bidentatae (RAB) and Radix Cyathulae (RC) are from two different medicinal plants, they are both used as 'Niu-Xi', a widely used traditional Chinese medicine that is believed to stimulate menstruation and affect bone injury. Angiogenesis is actively involved in treating these illnesses. The aim of the present study was to investigate whether the whole extracts of RAB and RC possess pro-angiogenic effects. In order to examine this idea whole extracts of RAB and RC were extracted with boiling water followed by ethanol, respectively. Results from the MTT, wound healing and tube formation assays in human umbilical vein endothelial cells (HUVECs) in vitro revealed that the whole extracts of RAB and RC did not increase cell proliferation or tube formation, but enhanced cell migration. Their angiogenic effects were also confirmed in zebrafish in vivo via increasing the sprout numbers in the sub-intestinal vessel. As determined by quantitative polymerase chain reaction, the whole extracts of RAB and RC both regulated the expression of cell migration-related genes in zebrafish. It is concluded that the whole extracts of RAB and RC induced angiogenesis in HUVECs in vitro and in zebrafish in vivo via increasing cell migration.
著者Zhou XL, Siu WS, Zhang C, Liu CL, Cheng L, Kwok HF, Fung CH, Tam JCW, Lau CP, Lau CBS, Leung PC, Hung LK, Ko CH
期刊名稱Experimental and Therapeutic Medicine
出版年份2017
月份3
卷號13
期次3
出版社SPANDIDOS PUBL LTD
頁次1032 - 1038
國際標準期刊號1792-0981
電子國際標準期刊號1792-1015
語言英式英語
關鍵詞Radix Achyranthis Bidentatae,radix cyathulae,pro-angiogenesis,zebrafish,cell migration
Web of Science 學科類別Medicine, Research & Experimental;Research & Experimental Medicine

上次更新時間 2020-27-10 於 03:06