Whole extracts of Radix Achyranthis Bidentatae and Radix Cyathulae promote angiogenesis in human umbilical vein endothelial cells in vitro and in zebrafish in vivo
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AbstractAlthough Radix Achyranthis Bidentatae (RAB) and Radix Cyathulae (RC) are from two different medicinal plants, they are both used as 'Niu-Xi', a widely used traditional Chinese medicine that is believed to stimulate menstruation and affect bone injury. Angiogenesis is actively involved in treating these illnesses. The aim of the present study was to investigate whether the whole extracts of RAB and RC possess pro-angiogenic effects. In order to examine this idea whole extracts of RAB and RC were extracted with boiling water followed by ethanol, respectively. Results from the MTT, wound healing and tube formation assays in human umbilical vein endothelial cells (HUVECs) in vitro revealed that the whole extracts of RAB and RC did not increase cell proliferation or tube formation, but enhanced cell migration. Their angiogenic effects were also confirmed in zebrafish in vivo via increasing the sprout numbers in the sub-intestinal vessel. As determined by quantitative polymerase chain reaction, the whole extracts of RAB and RC both regulated the expression of cell migration-related genes in zebrafish. It is concluded that the whole extracts of RAB and RC induced angiogenesis in HUVECs in vitro and in zebrafish in vivo via increasing cell migration.
All Author(s) ListZhou XL, Siu WS, Zhang C, Liu CL, Cheng L, Kwok HF, Fung CH, Tam JCW, Lau CP, Lau CBS, Leung PC, Hung LK, Ko CH
Journal nameExperimental and Therapeutic Medicine
Year2017
Month3
Volume Number13
Issue Number3
PublisherSPANDIDOS PUBL LTD
Pages1032 - 1038
ISSN1792-0981
eISSN1792-1015
LanguagesEnglish-United Kingdom
KeywordsRadix Achyranthis Bidentatae,radix cyathulae,pro-angiogenesis,zebrafish,cell migration
Web of Science Subject CategoriesMedicine, Research & Experimental;Research & Experimental Medicine

Last updated on 2020-24-05 at 00:54