Interaction between bacterial cell membranes and nano-TiO2 revealed by two-dimensional FTIR correlation spectroscopy using bacterial ghost as a model cell envelope
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AbstractThe interaction between microorganisms and nanoparticles is a crucial step towards understanding the subsequent biological effect. In this study, the interaction between TiO2 nanoparticles and bacterial cell membrane was investigated by Two-dimensional Correlation Fourier Transformation Infrared spectroscopy (2D-FTIR-COS) using bacterial ghosts (BGs), which are non-living bacterial cell envelopes devoid of cytoplasm. The synchronous map of 2D-FTIR-COS results indicated that the functionalities in proteins of BGs preferentially interacted with TiO2 nanoparticles; whereas the interaction of TiO2 nanoparticles with characteristic functionality in polysaccharides (CeOH) and phospholipids (P]O) were very weak or insensitive. This conclusion was further corroborated by settling of TiO2 nanoparticles in the presence of pure protein, polysaccharide and phospholipid represented by bovine serum albumin (BSA), alginate and phosphatidylethanolamine (PE). Additionally, the asynchronous map of 2D-FTIR-COS indicated a sequential order of functionalities bonded to TiO2 nanoparticles with the order of: COO > aromatic C]C stretching > NeH, amide II > C]O, ketone. These findings contribute to deeper understanding of the interaction between TiO2 nanoparticles and bacterial cell membrane in aquatic systems.
All Author(s) ListHUANG Guocheng, NG Tsz Wai, AN Taicheng, LI Guiying, WANG Bo, WU Dan, YIP Ho Yin, ZHAO Huijun Zhao, WONG Po Keung
Journal nameWater Research
Year2017
Month7
Day1
Volume Number118
PublisherElsevier
Place of PublicationNew York
Pages104 - 113
ISSN0043-1354
LanguagesEnglish-United States
KeywordsTitanium dioxide nanoparticles, Bacterial ghosts, Two-dimensional FTIR correlation, Nanoparticle-cell membrane interaction

Last updated on 2020-21-11 at 01:44