High-speed focal plane control via a liquid-crystal spatial light modulator for light sheet microscopy
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AbstractWe present a new method to perform high-speed axial scanning for selective plane illumination microscopy (SPIM) using a liquid crystal-spatial light modulator (LC-SLM). In our system, translation of the detection focal plane is achieved by projecting the emissions to the back of the galvanometric mirror, which subsequently scans the emission beam across an LC-SLM, where different regions are coded with designed phase modulating patterns. Accordingly, the transverse scanning performed by the galvanometric scanner is effectively converted to axial scanning. Synchronization is automatically achieved as two sides of the galvanometric mirror are simultaneously used to scan the illumination and emission beams. Practically, axial scanning speed is equivalent to the speed of the galvanometric scanner, i.e., 10s kHz, which enables video-rate volumetric imaging for SPIM.
All Author(s) ListChenyang Wen, Yina Chang, Chenglin Gu, Shih-Chi Chen
Name of ConferenceSPIE Photonics West Bios
Start Date of Conference28/01/2017
End Date of Conference02/02/2017
Place of ConferenceSan Francisco, California
Country/Region of ConferenceUnited States of America
LanguagesEnglish-United States
KeywordsMicroscopy, Three-dimensional microscopy, Active or adaptive optics

Last updated on 2018-22-01 at 05:54