Green tea polyphenols decreases uric acid level through xanthine oxidase and renal urate transporters in hyperuricemic mice
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摘要Ethnopharmacological relevance: Green tea is a Chinese materia medica with the main functions of "inducing urination and quenching thirst". Green tea polyphenols (GTP) are generally acknowledged as the main active fraction with multiple pharmacological functions in green tea. However, the effect of GTP on hyperuricemia is not clear till now.Aim of study: The present study was carried out to investigate the effect of GTP on serum level of uric acid in potassium oxonate (PO)-induced hyperuricemic mice, and explore the underlying mechanisms from two aspects of production and excretion of uric acid.Materials and methods: PO and GTP were intragastricly administered to mice for consecutive 7 days. Serum level of uric acid, and xanthine oxidase (XOD) activity in serum and liver were examined. Simultaneously, expression of XOD protein in liver was analyzed by Western blot assay. Expressions of urate transporters including urate-anion transporter (URAT) 1, organic anion transporter (OAT) 1 and 3 in kidney were analyzed by immunohistochemistry staining method.Results: 300 and 600 mg/kg GTP significantly decreased serum level of uric acid of hyperuricemic mice in a dose-dependent manner (p < 0.05 or p < 0.01). Besides, 300 and 600 mg/kg GTP markedly reduced XOD activity in serum and liver of hyperuricemic mice (both p < 0.01). Furthermore, 300 and 600 mg/kg GTP clearly reduced XOD expression in liver, as well as reduced URAT1 expression and increased OAT1 and OAT3 expressions in kidney of hyperuricemic mice (p < 0.05 or p < 0.01).Conclusions: These results demonstrated that GTP had the effect of lowering uric acid through decreasing the uric acid production and increasing uric acid excretion. Our study suggested that GTP would be a promising candidate as a novel hypouricaemic agent for further investigation.
著者Chen G, Tan ML, Li KK, Leung PC, Ko CH
期刊名稱Journal of Ethnopharmacology
出版年份2015
月份12
日期4
卷號175
出版社ELSEVIER IRELAND LTD
頁次14 - 20
國際標準期刊號0378-8741
語言英式英語
關鍵詞Green tea polyphenols,Xanthine oxidase,Urate transporters,Hyperuricemia
Web of Science 學科類別Plant Sciences;Chemistry, Medicinal;Integrative & Complementary Medicine;Pharmacology & Pharmacy;Plant Sciences;Pharmacology & Pharmacy;Integrative & Complementary Medicine

上次更新時間 2020-05-07 於 00:29