Berberine may rescue Fusobacterium nucleatum-induced colorectal tumorigenesis by modulating the tumor microenvironment
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AbstractBackground: Accumulating evidence links colorectal cancer (CRC) with the intestinal microbiota. However, the disturbance of intestinal microbiota and the role of Fusobacterium nucleatum during the colorectal adenoma-carcinoma sequence have not yet been evaluated. Methods: 454 FLX pyrosequencing was used to evaluate the disturbance of intestinal microbiota during the adenoma-carcinoma sequence pathway of CRC. Intestinal microbiota and mucosa tumor-immune cytokines were detected in mice after introducing 1,2-dimethylhydrazine (DMH), F. nucleatum or Berberine (BBR), using pyrosequencing and Bio-Plex Pro™ cytokine assays, respectively. Protein expressions were detected by western blotting. Results: The levels of opportunistic pathogens, such as Fusobacterium, Streptococcus and Enterococcus spp. gradually increased during the colorectal adenoma-carcinoma sequence in human fecal and mucosal samples. F. nucleatum treatment significantly altered lumen microbial structures, with increased Tenericutes and Verrucomicrobia (opportunistic pathogens) (P < 0.05 = in wild-type C57BL/6 and mice with DMH treatment). BBR intervention reversed the F. nucleatum-mediated increase in opportunistic pathogens, and the secretion of IL-21/22/31, CD40L and the expression of p-STAT3, p-STAT5 and p-ERK1/2 in mice, compared with mice fed with F. nucleatum alone. Conclusions: F. nucleatum colonization in the intestine may prompt colorectal tumorigenesis. BBR could rescue F. nucleatum-induced colorectal tumorigenesis by modulating the tumor microenvironment and blocking the activation of tumorigenesisrelated pathways.
All Author(s) ListYu Y.-N., Yu T.-C., Zhao H.-J., Sun T.-T., Chen H.-M., Chen H.-Y., An H.-F., Weng Y.-R., Yu J., Li M., Qin W.-X., Ma X., Shen N., Hong J., Fang J.-Y.
Journal nameOncotarget
Year2015
Month1
Day1
Volume Number6
Issue Number31
PublisherImpact Journals
Place of PublicationUnited States
Pages32013 - 32026
ISSN1949-2553
LanguagesEnglish-United Kingdom
KeywordsBerberine, Colorectal tumorigenesis, Fusobacterium nucleatum, Intestinal microbiota, Tumor-immune cytokine

Last updated on 2020-21-11 at 01:35