A traditional Chinese formula composed of Chuanxiong Rhizoma and Gastrodiae Rhizoma (Da Chuanxiong Formula) suppresses inflammatory response in LPS -induced RAW 264.7 cells through inhibition of NF-kappa B pathway
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摘要Ethnopharmacological relevance: Da Chuanxiong Formula (DCXF) which origins from Jin Dynasty is a famous classical 2-herb Chinese medicinal prescription. It is composed of dried rhizomes of Ligusticum chuanxiong (Chuanxiong Rhizoma, CR) and Gastrodia elata (Gastrodiae Rhizoma, GR) at the ratio of 4:1 (w/w). It has been used to treat headache which is caused by wind pathogen and blood stasis for thousands of years in China.Aim of study: The present study was performed to investigate the anti-inflammatory effect of DCXF and elucidate its underlying molecular mechanisms using LPS-stimulated RAW 264.7 cells.Materials and methods: The anti-inflammatory effect of DCXF was evaluated using LPS-stimulated RAW 264.7 cells. Generation of nitric oxide (NO) and prostaglandin E-2 (PGE(2)) were measured by the Griess colorimetric method and enzyme-linked immunosorbent assay (ELISA), respectively. The gene expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were detected by reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, the effect of DCXF on NF-kappa B activation was measured by western blot assay.Results: Treatment with DCXF significantly suppressed the productions of NO and PGE(2) through inhibitions of iNOS and COX-2 expressions in LPS-stimulated RAW 264.7 cells. DCXF significantly decreased I kappa B alpha phosphorylation, inhibited p65 expression and reduced p-p65 level. These results suggested the anti-inflammatory effect of DCXF was associated with the reduction of inflammatory mediators through inhibition of NF-kappa B pathway.Conclusions: These results indicated that DCXF inhibited inflammation in LPS-stimulated RAW 264.7 cells through inactivation of NF-kappa B pathway.
出版社接受日期10.12.2016
著者Liu ZK, Ng CF, Shiu HT, Wong HL, Wong CW, Li KK, Zhang JF, Lam PK, Poon WS, Lau CBS, Leung PC, Ko CH
期刊名稱Journal of Ethnopharmacology
出版年份2017
月份1
日期20
卷號196
出版社ELSEVIER IRELAND LTD
頁次20 - 28
國際標準期刊號0378-8741
語言英式英語
關鍵詞Chuanxiong Rhizoma,Gastrodiae Rhizoma,Da Chuanxiong Formula,Anti-inflammation,NF-kappa B
Web of Science 學科類別Plant Sciences;Chemistry, Medicinal;Integrative & Complementary Medicine;Pharmacology & Pharmacy;Plant Sciences;Pharmacology & Pharmacy;Integrative & Complementary Medicine

上次更新時間 2020-20-10 於 03:19