Enhancing cartilage repair with optimized supramolecular hydrogel-based scaffold and pulsed electromagnetic field
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AbstractFunctional tissue engineering strategies provide innovative approach for the repair and regeneration of damaged
cartilage. Hydrogel is widely used because it could provide rapid defect filling and proper structure support, and
is biocompatible for cell aggregation and matrix deposition. Efforts have been made to seek suitable scaffolds for
cartilage tissue engineering. Here Alg-DA/Ac-β-CD/gelatin hydrogel was designed with the features of physical
and chemical multiple crosslinking and self-healing properties. Gelation time, swelling ratio, biodegradability
and biocompatibility of the hydrogels were systematically characterized, and the injectable self-healing adhesive
hydrogel were demonstrated to exhibit ideal properties for cartilage repair. Furthermore, the new hydrogel
design introduces a pre-gel state before photo-crosslinking, where increased viscosity and decreased fluidity
allow the gel to remain in a semi-solid condition. This granted multiple administration routes to the hydrogels,
which brings hydrogels the ability to adapt to complex clinical situations. Pulsed electromagnetic fields (PEMF)
have been recognized as a promising solution to various health problems owing to their noninvasive properties
and therapeutic potentials. PEMF treatment offers a better clinical outcome with fewer, if any, side effects, and
wildly used in musculoskeletal tissue repair. Thereby we propose PEMF as an effective biophysical stimulation to
be 4th key element in cartilage tissue engineering. In this study, the as-prepared Alg-DA/Ac-β-CD/gelatin
hydrogels were utilized in the rat osteochondral defect model, and the potential application of PEMF in cartilage
tissue engineering were investigated. PEMF treatment were proven to enhance the quality of engineered chondrogenic constructs in vitro, and facilitate chondrogenesis and cartilage repair in vivo. All of the results suggested
that with the injectable self-healing adhesive hydrogel and PEMF treatment, this newly proposed tissue engineering
strategy revealed superior clinical potential for cartilage defect treatment.
cartilage. Hydrogel is widely used because it could provide rapid defect filling and proper structure support, and
is biocompatible for cell aggregation and matrix deposition. Efforts have been made to seek suitable scaffolds for
cartilage tissue engineering. Here Alg-DA/Ac-β-CD/gelatin hydrogel was designed with the features of physical
and chemical multiple crosslinking and self-healing properties. Gelation time, swelling ratio, biodegradability
and biocompatibility of the hydrogels were systematically characterized, and the injectable self-healing adhesive
hydrogel were demonstrated to exhibit ideal properties for cartilage repair. Furthermore, the new hydrogel
design introduces a pre-gel state before photo-crosslinking, where increased viscosity and decreased fluidity
allow the gel to remain in a semi-solid condition. This granted multiple administration routes to the hydrogels,
which brings hydrogels the ability to adapt to complex clinical situations. Pulsed electromagnetic fields (PEMF)
have been recognized as a promising solution to various health problems owing to their noninvasive properties
and therapeutic potentials. PEMF treatment offers a better clinical outcome with fewer, if any, side effects, and
wildly used in musculoskeletal tissue repair. Thereby we propose PEMF as an effective biophysical stimulation to
be 4th key element in cartilage tissue engineering. In this study, the as-prepared Alg-DA/Ac-β-CD/gelatin
hydrogels were utilized in the rat osteochondral defect model, and the potential application of PEMF in cartilage
tissue engineering were investigated. PEMF treatment were proven to enhance the quality of engineered chondrogenic constructs in vitro, and facilitate chondrogenesis and cartilage repair in vivo. All of the results suggested
that with the injectable self-healing adhesive hydrogel and PEMF treatment, this newly proposed tissue engineering
strategy revealed superior clinical potential for cartilage defect treatment.
Acceptance Date05/10/2022
All Author(s) ListYucong Li, Linlong Li, Ye Li, Lu Feng, Bin Wang, Ming Wang, Haixing Wang, Meiling Zhu, Yongkang Yang, Erik I. Waldorff, Nianli Zhang, Ingmar Viohl, Sien Lin, Liming Bian, Wayne Yuk-Wai Lee, Gang Li
Journal nameBioactive Materials
Year2023
Month4
Volume Number22
Pages312 - 324
eISSN2452-199X
LanguagesEnglish-United States
