Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要Consumption of fruits and vegetables is generally regarded as beneficial to plasma lipid profile. The mechanism by which the plant foods induce desirable lipid changes remains unclear. SREBP-2 is crucial in cholesterol metabolism, and it is a major regulator of the cholesterol biosynthesis enzyme HMGCR. Our lab has previously illustrated that apigenin and luteolin could attenuate the nuclear translocation of SREBP-2 through an AMPK-dependent pathway. In the present study, these two flavones were studied for their ability to deter the same in an AMPK-independent signaling route. The processing of SREBP-2 protein was promoted by phorbol 12-myristate 13-acetate (PMA) in the hepatic cells WRL and HepG2, and the increased processing was reversed by apigenin or luteolin co-administration. EMSA results demonstrated that the PMA-induced DNA-binding activity was weakened by the flavones. The increased amount of nuclear SREBP-2 in cells was attenuated by the flavonoid as shown by immunocytochemical imaging. Quantitative reverse transcriptase-polymerase chain reaction assay demonstrated that the transcription of HMGCR under both flavone treatments was reduced. However, apigenin appeared to be stronger than luteolin in restraining PMA-induced HMGCR mRNA expression. Since PMA is a diacylglycerol analog, these findings might have some physiological implications.
著者Tan Yan Qin, Wong Tsz Yan, Lin Shu Mei, Leung Lai Kwok
期刊名稱Molecular and Cellular Biochemistry
出版年份2017
月份1
日期1
卷號424
期次1-2
出版社SPRINGER
頁次163 - 172
國際標準期刊號0300-8177
電子國際標準期刊號1573-4919
語言英式英語
關鍵詞Apigenin,Luteolin,PKC-dependent pathway,SREBP-2,HMGCR,Nuclear translocation
Web of Science 學科類別Cell Biology;Cell Biology

上次更新時間 2020-20-11 於 02:22