Automated optical tweezers manipulation to transfer mitochondria from fetal to adult mscs to improve antiaging gene expressions
Publication in policy or professional journal
CUHK Authors
Full Text
Digital Object Identifier (DOI) DOI for CUHK Users |
Times Cited
Altmetrics Information
.
Other information
AbstractMitochondrial dysfunction is considered to be an important factor that leads to aging and premature aging diseases. Transferring mitochondria to cells is an emerging and promising technique for the therapy of mitochondrial deoxyribonucleic acid (mtDNA)-related diseases. This paper presents a unique method of controlling the quality and quantity of mitochondria transferred to single cells using an automated optical tweezer-based micromanipulation system. The proposed method can automatically, accurately, and efficiently collect and transport healthy mitochondria to cells, and the recipient cells then take up the mitochondria through endocytosis. The results of the study reveal the possibility of using mitochondria from fetal mesenchymal stem cells (fMSCs) as a potential source to reverse the aging-related phenotype and improve metabolic activities in adult mesenchymal stem cells (aMSCs). The results of the quantitative polymerase chain reaction analysis show that the transfer of isolated mitochondria from fMSCs to a single aMSC can significantly increase the antiaging and metabolic gene expression in the aMSC. The proposed mitochondrial transfer method can greatly promote precision medicine for cell therapy of mtDNA-related diseases.
All Author(s) ListShakoor A, Wang B, Fan L, Kong L, Gao W, Sun J, Man K, Li G, Sun D
Journal nameSmall
Year2021
Month9
Day23
Volume Number17
Issue Number38
Publisherwiley
Article number2103086
ISSN1613-6810
LanguagesEnglish-United States
