The flame retardant 2,2′,4,4′-Tetrabromodiphenyl ether enhances the expression of corticotropin-releasing hormone in the placental cell model JEG-3
Publication in refereed journal


摘要Polybrominated diphenyl ethers (PBDEs) are chemicals used as flame retardants in household products. After disposing of these items, {PBDEs} leach from the products by surface water. BDE-47 is a {PBDE} congener commonly isolated from contaminated food and is the most studied isomer. The placenta is the major source of hormones during pregnancy, and an elevated level of corticotrophin-releasing hormone (CRH) is associated with premature delivery. In the present study, we examined changes in the placental {CRH} expression under BDE-47 exposure in the JEG-3 cell model system. These placental cells are derived from human choriocarcinoma. Our result showed that this pollutant induced the {CRH} mRNA expression at 0.5 nM or above in the cells. A similar trend was observed when {CRH} peptide was determined by Western analysis in the cell lysates. As previous studies have shown the importance of signal transduction pathways in the gene regulation, the status of some protein kinases in the present study was investigated. The phosphorylated PKCα, JNK, and {P38} were increased by the toxicant treatment, and administering the specific inhibitors could counteract the induced {CRH} expression. It appeared that the signaling transduction pathway of {PKC} was a significant contributor in the transcriptional regulation. Further study by using Electrophoretic Mobility Shift Assay suggested that AP-2 was the ultimate DNA-binding element for the initiation of gene transcription. Because an untimely increased {CRH} may compromise fetal development and induce preterm birth, the present study suggested that endocrine changes in pregnancy should be taken into consideration in the next assessment.
著者Zhu Yun, Tan Yan Qin, Wang Chi Chiu, Leung Lai K.
出版地England, United Kingdom
頁次499 - 505
關鍵詞BDE-47, CRH, Placental cells

上次更新時間 2020-13-10 於 03:07