3D-bioprinted BMSC-laden biomimetic multiphasic scaffolds for efficient repair of osteochondral defects in an osteoarthritic rat model
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香港中文大學研究人員

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其它資訊
摘要Osteochondral defect repair in osteoarthritis (OA) remains an unsolved clinical problem due to the lack of enough seed cells in the defect and chronic inflammation in the joint. To address this clinical need, we designed a bone marrow-derived mesenchymal stem cell (BMSC)-laden 3D-bioprinted multilayer scaffold with methacrylated hyaluronic acid (MeHA)/polycaprolactone incorporating kartogenin and β-TCP for osteochondral defect repair within each region. BMSC-laden MeHA was designed to actively introduce BMSCs in situ, and diclofenac sodium (DC)-incorporated matrix metalloproteinase-sensitive peptide-modified MeHA was induced on the BMSC-laden scaffold as an anti-inflammatory strategy. BMSCs in the scaffolds survived, proliferated, and produced large amounts of cartilage-specific extracellular matrix in vitro. The effect of BMSC-laden scaffolds on osteochondral defect repair was investigated in an animal model of medial meniscectomy-induced OA. BMSC-laden scaffolds facilitated chondrogenesis by promoting collagen II and suppressed interleukin 1β in osteochondral defects of the femoral trochlea. Congruently, BMSC-laden scaffolds significantly improved joint function of the injured leg with respect to the ground support force, paw grip force, and walk gait parameters. Therefore, this research demonstrates the potential of 3D-bioprinted BMSC-laden scaffolds to simultaneously inhibit joint inflammation and promote cartilage defect repair in OA joints.
出版社接受日期20.10.2021
著者Lin Y, Peng L, Li L, Huang C, Shi K, Meng X, Wang P, Wu M, Li L, Cao H, Wu K, Zeng Q, Pan H, Lu WW, QIn L, Ruan C, Wang X
期刊名稱Biomaterials
出版年份2021
月份12
卷號279
出版社Elsevier
文章號碼121216
國際標準期刊號0142-9612
電子國際標準期刊號1878-5905
語言美式英語
關鍵詞3D bioprinting, BMSC-Laden scaffolds, Cartilage defect

上次更新時間 2024-16-04 於 00:20