Upregulation of microRNA-96-5p is associated with adolescent idiopathic scoliosis and low bone mass phenotype
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摘要Bone densitometry revealed low bone mass in patients with adolescent idiopathic scoliosis (AIS) and its prognostic potential to predict curve progression. Recent studies showed differential circulating miRNAs in AIS but their diagnostic potential and links to low bone mass have not been well-documented. The present study aimed to compare miRNA profiles in bone tissues collected from AIS and non-scoliotic subjects, and to explore if the selected miRNA candidates could be useful diagnostic biomarkers for AIS. Microarray analysis identified miR-96-5p being the most upregulated among the candidates. miR-96-5p level was measured in plasma samples from 100 AIS and 52 healthy girls. Our results showed significantly higher plasma levels of miR-96-5p in AIS girls with an area under the curve (AUC) of 0.671 for diagnostic accuracy. A model that was composed of plasma miR-96-5p and patient-specific parameters (age, body weight and years since menarche) gave rise to an improved AUC of 0.752. Ingenuity Pathway Analysis (IPA) indicated functional links between bone metabolic pathways and miR-96-5p. In conclusion, differentially expressed miRNAs in AIS bone and plasma samples represented a new source of disease biomarkers and players in AIS etiopathogenesis, which required further validation study involving AIS patients of both genders with long-term follow-up.
出版社接受日期18.05.2022
著者Huanxiong Chen, Kenneth Guangpu Yang, Jiajun Zhang, Ka‑yee Cheuk, Evguenia Nepotchatykh, Yujia Wang, Alec Lik‑hang Hung, Tsz‑ping Lam, Alain Moreau, Wayne Yuk‑wai Lee
期刊名稱Scientific Reports
出版年份2022
月份6
卷號12
文章號碼9705
國際標準期刊號2045-2322
電子國際標準期刊號2045-2322
語言英式英語
