Acute Inflammatory Response in Osteoporotic Fracture Healing Augmented with Mechanical Stimulation is Regulated In Vivo through the p38-MAPK Pathway
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香港中文大學研究人員
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摘要Low-magnitude high-frequency vibration (LMHFV) has previously been reported to modulate the acute inflammatory response of ovariectomy-induced osteoporotic fracture healing. However, the underlying mechanisms are not clear. In the present study, we investigated the effect of LMHFV on the inflammatory response and the role of the p38 MAPK mechanical signaling pathway in macrophages during the healing process. A closed femoral fracture SD rat model was used. In vivo results showed that LMHFV enhanced activation of the p38 MAPK pathway at the fracture site. The acute inflammatory response, expression of inflammatory cytokines, and callus formation were suppressed in vivo by p38 MAPK inhibition. However, LMHFV did not show direct in vitro enhancement effects on the polarization of RAW264.7 macrophage from the M1 to M2 phenotype, but instead promoted macrophage enlargement and transformation to dendritic monocytes. The present study demonstrated that p38 MAPK modulated the enhancement effects of mechanical stimulation in vivo only. LMHFV may not have exerted its enhancement effects directly on macrophage, but the exact mechanism may have taken a different pathway that requires further investigation in the various subsets of immune cells.
出版社接受日期12.08.2021
著者Chow Ho Kwoon Simon, Cui Can, Cheng Kin Yu Keith, Chim Ning Yu, Wang Jinyu, Wong Wai Hing Carissa, Ng Wai Ka, Wong Yeung Man Ronald, Cheung Hoi Wing
期刊名稱International Journal of Molecular Sciences
出版年份2021
月份8
卷號22
期次16
文章號碼8720
國際標準期刊號1661-6596
語言美式英語
關鍵詞Inflammatory response, osteoporotic fracture healing, mechanical stimulation
