Idiopathic Autism: Cellular and Molecular Phenotypes in Pluripotent Stem Cell-derived Neurons
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AbstractAutism spectrum disorder is a complex neurodevelopmental disorder whose pathophysiology remains elusive as a consequence of the unavailability for study of patient brain neurons; this deficit may potentially be circumvented by neural differentiation of induced pluripotent stem cells. Rare syndromes with single gene mutations and autistic symptoms have significantly advanced the molecular and cellular understanding of autism spectrum disorders; however, in aggregate, they only represent a fraction of all cases of autism. In an effort to define the cellular and molecular phenotypes in human neurons of non-syndromic autism, we generated induced pluripotent stem cells (iPSCs) from three male autism spectrum disorder patients who had no identifiable clinical syndromes, and their unaffected male siblings and subsequently differentiated these patient-specific stem cells into electrophysiologically active neurons. iPSC-derived neurons from these autistic patients displayed decreases in the frequency and kinetics of spontaneous excitatory postsynaptic currents relative to controls, as well as significant decreases in Na+ and inactivating K+ voltage-gated currents. Moreover, whole-genome microarray analysis of gene expression identified 161 unique genes that were significantly differentially expressed in autistic patient iPSC-derived neurons (>twofold, FDR < 0.05). These genes were significantly enriched for processes related to synaptic transmission, such as neuroactive ligand-receptor signaling and extracellular matrix interactions, and were enriched for genes previously associated with autism spectrum disorder. Our data demonstrate aberrant voltage-gated currents and underlying molecular changes related to synaptic function in iPSC-derived neurons from individuals with idiopathic autism as compared to unaffected siblings controls.
All Author(s) ListLIU Xiaozhuo, CAMPANAC Emilie, CHEUNG Hoi Hung, ZIATS Mark N., CANTEREL-THOUENNON Lucile, RAYGADA Margarita, BAXENDALE Vanessa, PANG Alan Lap-yin, YANG Lu, SWEDO Susan, THURM Audrey, LEE Tin Lap, FUNG Kwok Pui, CHAN Wai Yee, HOFFMAN Dax A., RENNERT Owen M.
Journal nameMolecular Neurobiology
Volume Number54
Issue Number6
Pages4507 - 4523
LanguagesEnglish-United Kingdom
KeywordsAutism spectrum disorder (ASD), Gene expression, Induced pluripotent stem cell (iPSC)

Last updated on 2021-22-10 at 00:57