In Vivo and In Vitro Anti-Inflammatory Effects of Zao-Jiao-Ci (The Spine of Gleditsia Sinensis Lam.) aqueous Extract and Its Mechanisms of Action
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AbstractEthnopharmacological relevance Zao-Jiao-Ci (ZJC), as the spine of Chinese Honey locust (Gleditsia sinensis Lam.), is traditionally used as Chinese medicine to reduce inflammation. Aim of the study The present study aimed to investigate an anti-inflammatory effect of ZJC aqueous extract both in vitro and in vivo, as well as its underlying mechanisms. Materials and methods Anti-inflammatory effect of ZJC aqueous extract was evaluated by using carrageenan-induced paw edema in rats. In addition, the inhibitory effects of ZJC on nitric oxide production, intracellular reactive oxygen species production, pro-inflammatory mediator expression and prostaglandin E2 (PGE2) production were determined by using LPS-activated RAW 264.7 cells. The anti-oxidant activity of ZJC was assessed using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid assay. Results ZJC aqueous extract showed significant suppressive effect on paw edema in rats at 100 mg/kg. Moreover, ZJC aqueous extract decreased the expression of cyclooxygenase (COX)-2 and significantly decreased the PGE2, tumor necrosis factor-α, interleukin (IL)-1β and IL-6 production in LPS-activated macrophages in dose-dependent manners. ZJC aqueous extract inhibited the mRNA expression of these inflammatory cytokines as well. Furthermore, ZJC aqueous extract was found as an anti-oxidant and could inhibit ROS production in the LPS-induced cells. Conclusions These findings show the potential of ZJC aqueous extract as a naturally occurring COX-2 inhibitor to reduce inflammation.
All Author(s) ListLI Kaikai, ZHOU Xuelin, WONG Hing Lok, NG Chun Fai, FU Wei Ming, LEUNG Ping Chung, PENG Guiyuan, KO Chun Hay
Journal nameJournal of Ethnopharmacology
Volume Number192
PublisherElsevier Ireland Ltd.
Pages192 - 200
LanguagesEnglish-United Kingdom
KeywordsGleditsia sinensis Lam.; Anti-inflammation; Anti-oxidative

Last updated on 2021-24-09 at 23:43