An In Vitro Platform for Screening Chinese Herbal Medicines That Inhibit Cancer Stem-Like Cells in Breast Cancer
Refereed conference paper presented and published in conference proceedings


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AbstractIntroduction: With the high recurrence rate of breast cancer, cancer stem-like cells (CSCs) are believed to be one of the possible cause of cancer recurrence. CSCs are subpopulation among cancer cells that possess stem cell characteristics: self-renewal and differentiation, and responsible for tumour’s cellular heterogeneity. Conventional therapies usually target on growing cancer cells rather than CSCs, so cancer recurrence may occur. The present study aimed to establish an in vitro platform for screening Chinese herbal medicines or natural products that inhibit the activities of CSCs. Methods: Breast CSCs-enriched cancer cells were developed from parental human breast cancer cell line MDA-MB-231 using two cell culture conditions: (1) culture medium supplemented with various growth factors particularly for CSCs (specific stem cell medium); (2) culture medium and low dose of doxorubicin. Results: The use of specific stem cell medium is preferred than that of culture medium and low dose of doxorubicin, since CSCs-enriched MDA-MB-231 cells exhibited higher self-renewal abilities and tumorigenicity, as compared with parental MDA-MB-231 cells. Our results also demonstrated that Chinese herb Hedyotis dif usa and its isolated compound inhibited spheroid formation of CSCs-enriched MDA-MB-231 cells. Conclusions: This screening platform can be applied to identify Chinese herbal medicines as potential anti-cancer agents that inhibit the activities of CSCs.
All Author(s) ListLEUNG Hoi Wing, KO Chun Hay, YUE Gar Lee Grace, LAU Bik San Clara
Name of Conference5th Annual Meeting of GP-TCM Research Association-cum-Summit on Compendium of Materia Medica and Innovative Drug Discovery in Chinese Medicine
Start Date of Conference09/08/2016
End Date of Conference10/08/2016
Place of ConferenceHong Kong
Country/Region of ConferenceChina
Proceedings Title5th Annual Meeting of GP-TCM Research Association-cum-Summit on Compendium of Materia Medica and Innovative Drug Discovery in Chinese Medicine
Year2016
Month8
Day9
Place of PublicationChina, Hong Kong
Pages1
LanguagesEnglish-United Kingdom

Last updated on 2018-22-01 at 15:44