Anti-Angiogenic and Immunomodulatory Activities of a Labdane Diterpene from Leonurus Heterophyllus
Refereed conference paper presented and published in conference proceedings


其它資訊
摘要Introduction: Heteronone B (HB) is a labdane diterpene isolated from Leonurus heterophyllus Sweet which is known as Yimucao in Chinese medicines and is traditionally used for the treatment of gynaecological problems. HB was firstly isolated a decade ago but its biological activity has never been reported. Hence, the objectives of this study were to investigate the effects of HB on angiogenesis and immunomodulation using zebrafish embryos and human peripheral blood mononuclear cells (PBMCs), respectively. Methods: Angiogenesis in transgenic zebrafish Tg(fli1:EGFP)y1 was assessed by measuring the length of sub-intestinal vessels of embryos treated with HB. The mRNA expressions of angiogenic receptors in HB-treated zebrafish embryos were also examined. Besides, the roliferation and cytokines productions of PBMCs treated with HB were determined by thymidine incorporation assay and ELISA, respectively. Results: HB (5-10 uM) was shown to significantly inhibit the growth of sub-intestinal vessels of zebrafish embryos without observed toxicity. In addition, it could down-regulate the mRNA expressions of angiogenesis-related genes in zebrafish embryos. Results on human PBMCs showed that HB (6.25-50 uM) suppressed the proliferation of phytohaemagglutinin (PHA)-activated PBMCs. The levels of IL-10, IL-12, TNF-
and IFN- were modulated in PHA-activated PBMCs after HB treatment for 24 hours. Conclusions: This is the first report of the biological activities of HB showing the anti-angiogenic activities in zebrafish embryos as well as immunomodulatory activities in PBMCs. Our findings indicate that heteronone B may also act as an active component in this herb.
著者YUE Gar Lee Grace, LEE Kin Ming, KWOK Hin Fai, WU Da Rong, LIU Shao Hua, XIAO Wei Lie, LAU Bik San Clara
會議名稱5th Annual Meeting of GP-TCM Research Association-cum-Summit on Compendium of Materia Medica and Innovative Drug Discovery in Chinese Medicine
會議開始日09.08.2016
會議完結日10.08.2016
會議地點Hong Kong
會議國家/地區中國
會議論文集題名5th Annual Meeting of GP-TCM Research Association-cum-Summit on Compendium of Materia Medica and Innovative Drug Discovery in Chinese Medicine
出版年份2016
月份8
日期9
出版地China, Hong Kong
頁次1
語言英式英語

上次更新時間 2018-22-01 於 14:17