Anti-Angiogenic and Immunomodulatory Activities of a Labdane Diterpene from Leonurus Heterophyllus
Refereed conference paper presented and published in conference proceedings


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AbstractIntroduction: Heteronone B (HB) is a labdane diterpene isolated from Leonurus heterophyllus Sweet which is known as Yimucao in Chinese medicines and is traditionally used for the treatment of gynaecological problems. HB was firstly isolated a decade ago but its biological activity has never been reported. Hence, the objectives of this study were to investigate the effects of HB on angiogenesis and immunomodulation using zebrafish embryos and human peripheral blood mononuclear cells (PBMCs), respectively. Methods: Angiogenesis in transgenic zebrafish Tg(fli1:EGFP)y1 was assessed by measuring the length of sub-intestinal vessels of embryos treated with HB. The mRNA expressions of angiogenic receptors in HB-treated zebrafish embryos were also examined. Besides, the roliferation and cytokines productions of PBMCs treated with HB were determined by thymidine incorporation assay and ELISA, respectively. Results: HB (5-10 uM) was shown to significantly inhibit the growth of sub-intestinal vessels of zebrafish embryos without observed toxicity. In addition, it could down-regulate the mRNA expressions of angiogenesis-related genes in zebrafish embryos. Results on human PBMCs showed that HB (6.25-50 uM) suppressed the proliferation of phytohaemagglutinin (PHA)-activated PBMCs. The levels of IL-10, IL-12, TNF-
and IFN- were modulated in PHA-activated PBMCs after HB treatment for 24 hours. Conclusions: This is the first report of the biological activities of HB showing the anti-angiogenic activities in zebrafish embryos as well as immunomodulatory activities in PBMCs. Our findings indicate that heteronone B may also act as an active component in this herb.
All Author(s) ListYUE Gar Lee Grace, LEE Kin Ming, KWOK Hin Fai, WU Da Rong, LIU Shao Hua, XIAO Wei Lie, LAU Bik San Clara
Name of Conference5th Annual Meeting of GP-TCM Research Association-cum-Summit on Compendium of Materia Medica and Innovative Drug Discovery in Chinese Medicine
Start Date of Conference09/08/2016
End Date of Conference10/08/2016
Place of ConferenceHong Kong
Country/Region of ConferenceChina
Proceedings Title5th Annual Meeting of GP-TCM Research Association-cum-Summit on Compendium of Materia Medica and Innovative Drug Discovery in Chinese Medicine
Year2016
Month8
Day9
Place of PublicationChina, Hong Kong
Pages1
LanguagesEnglish-United Kingdom

Last updated on 2018-22-01 at 14:17