Expression of Antioxidant Molecules and Heat Shock Protein 27 in Thyroid Tumors
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摘要Oxidative stress-induced DNA damage is a known causing factor for many types of tumors, but information on the role of oxidants and antioxidants in thyroid tumors is limited. The aim of this study was to determine antioxidant levels in thyroid tumors. In this study, tumor and its matched non-tumor thyroid tissue samples were obtained from 53 patients with thyroid tumors. The levels of manganese superoxide dismutase (MnSOD), thioredoxin reductase 2 (TXNRD2), glutathione (GSH), glutathione peroxidase (Gpx), catalase (CAT), and 27kd heat-shock protein (hsp27) were determined in both thyroid tissue samples and cultured thyroid cells by immunohistochemical staining and western blot. Hydrogen peroxide (H2O2) was used to generate oxidant stress in the cell culture experiments. We found that the levels of MnSOD, TXNRD2, GSH, Gpx, and Hsp27 were increased in both malignant and benign tumors, while the level of CAT was decreased. To verify the results of the tissue study, we treated cultured thyroid cells with H2O2 and found the same pattern of antioxidant changes. Hsp27 was also increased after H2O2 treatment. The expression of hsp27 was upregulated by 8.24-, 6.96-, and 3.09-fold in thyroid cancer, follicular adenoma, multinodular goiter, respectively. Collectively, our study demonstrated that the levels of hsp27 together with MnSOD, TXNRD2, GSH, and Gpx were significantly upregulated by H2O2 in thyroid tumors. The increase of these antioxidants is observed in both malignant and benign tumors, particularly in the former. The upregulation of antioxidants is likely a protective mechanism of tumor cells to maintain their survival and growth. J. Cell. Biochem. 117: 2473-2481, 2016. (c) 2016 Wiley Periodicals, Inc.
著者Wang SS, Yang SC, Vlantis AC, Liu SYW, Ng EKW, Chan ABW, Wu J, Du J, Wei W, Liu XL, Liu ZM, Ng SK, van Hasselt CA, Tong MCF, Chen GG
期刊名稱Journal of Cellular Biochemistry
出版年份2016
月份11
日期1
卷號117
期次11
出版社WILEY-BLACKWELL
頁次2473 - 2481
國際標準期刊號0730-2312
電子國際標準期刊號1097-4644
語言英式英語
關鍵詞27kd HEAT-SHOCK PROTEIN; ANTIOXIDANTS; OXIDANTS; THYROID TUMORS
Web of Science 學科類別Biochemistry & Molecular Biology; Cell Biology

上次更新時間 2020-22-11 於 00:12