Epigenetic identification, of ZNF545 as a functional tumor suppressor in multiple myeloma via activation of p53 signaling pathway
Publication in refereed journal

香港中文大學研究人員

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摘要The KRAB zinc-finger protein ZNF545 was recently identified as a potential suppressor gene in several tumors. However, the regulatory mechanisms of ZNF545 in tumorigenesis remain unclear. In this study, we investigated the expression and roles of ZNF545 in multiple myeloma (MM). ZNF545 was frequently downregulated in MM tissues compared with non-tumor bone marrow tissues. ZNF545 expression was silenced by promoter methylation in MM cell lines, and could be restored by demethylation treatment. ZNF545 methylation was detected in 28.3% of MM tissues, compared with 4.3% of normal bone marrow tissues. ZNF545 transcriptionally activated the p53 signaling pathway but had no effect on Akt in MM, whereas ectopic expression of ZNF545 in silenced cells suppressed their proliferation and induced apoptosis. We therefore identified ZNF545 as a novel tumor suppressor inhibiting tumor growth through activation of the p53 pathway in MM. Moreover, tumor-specific methylation of ZNF545 may represent an epigenetic biomarker for MM diagnosis, and a potential target for specific therapy. (C) 2016 Elsevier Inc. All rights reserved.
著者Fan Y, Zhan Q, Xu HY, Li LL, Li C, Xiao Q, Xiang SL, Hui TL, Xiang TX, Ren GS
期刊名稱Biochemical and Biophysical Research Communications,Biochemical and Biophysical Research Communications
出版年份2016
月份6
日期10
卷號474
期次4
出版社ACADEMIC PRESS INC ELSEVIER SCIENCE
頁次660 - 666
國際標準期刊號0006-291X
電子國際標準期刊號1090-2104
語言英式英語
關鍵詞Methylation; Multiple myeloma; Tumor suppressor; ZNF545
Web of Science 學科類別Biochemistry & Molecular Biology; Biophysics

上次更新時間 2020-20-11 於 02:12