Role of TRPV1 in the Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes
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AbstractCytosolic Ca2+ ([Ca2+](i)) is an important signal that regulates cardiomyocyte differentiation during cardiogenesis. TRPV1 is a Ca2+-permeable channel that is expressed in cardiomyocytes. In the present study, we utilized mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs) as a model to investigate the functional role of TRPV1 in cardiomyocyte differentiation. Induction of embryonic stem cells into cardiomyocytes was achieved using embryoid body (EB)-based differentiation method. Quantitative PCRs showed an increased TRPV1 expression during the differentiation process. In [Ca2+](i) measurement study, application of TRPV1 agonists, capsaicin and camphor, elicited a [Ca2+](i) rise in mESC-CMs, the effect of which was abolished by TRPV1-shRNA. In functional study, treatment of EBs with TRPV1 antagonists (capsazepine and SB366791) and TRPV1-shRNA reduced the size of the EBs and decreased the percentage of spontaneously beating EBs. TRPV1 antagonists and TRPV1-shRNA also suppressed the expression of cardiomyocyte marker genes, including cardiac actin, c-TnT, c-TnI, and alpha-MHC. Taken together, this study demonstrated an important functional role of TRPV1 channels in the differentiation of mESCs into cardiomyocytes.
All Author(s) ListQi Y, Qi ZH, Li ZC, Wong CK, So C, Lo IC, Huang Y, Yao XQ, Tsang SY
Journal namePLoS ONE
Detailed descriptionTo ORKTS: Prof. Tsang and Prof. Yao are co-corresponding authors.
Year2015
Month7
Day24
Volume Number10
Issue Number7
PublisherPUBLIC LIBRARY SCIENCE
ISSN1932-6203
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesMultidisciplinary Sciences; Science & Technology - Other Topics

Last updated on 2020-25-09 at 14:40