Curcumin-conjugated magnetic nanoparticles for detecting amyloid plaques in Alzheimer's disease mice using magnetic resonance imaging (MRI)
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AbstractDiagnosis of Alzheimer's disease (AD) can be performed with the assistance of amyloid imaging. The current method relies on positron emission tomography (PET), which is expensive and exposes people to radiation, undesirable features for a population screening method. Magnetic resonance imaging (MRI) is cheaper and is not radioactive. Our approach uses magnetic nanoparticles (MNPs) made of superparamagnetic iron oxide (SPIO) conjugated with curcumin, a natural compound that specifically binds to amyloid plaques. Coating of curcumin-conjugated MNPs with polyethylene glycol-polylactic acid block copolymer and polyvinylpyrrolidone by antisolvent precipitation in a multi-inlet vortex mixer produces stable and biocompatible curcumin magnetic nanoparticles (Cur-MNPs) with mean diameter <100 nm. These nanoparticles were visualized by transmission electron microscopy and atomic force microscopy, and their structure and chemistry were further characterized by X-ray diffraction, thermogravimetric analysis, X-ray photoelectron spectroscopy, time-of-flight secondary ion mass spectrometry, and Fourier transform infrared spectroscopy. Cur-MNPs exhibited no cytotoxicity in either Madin-Darby canine kidney (MDCK) or differentiated human neuroblastoma cells (SH-SY5Y). The P-app of Cur-MNPs was 1.03 x 10(-6) cm/s in an in vitro blood brain barrier (BBB) model. Amyloid plaques could be visualized in ex vivo T2*-weighted magnetic resonance imaging (MRI) of Tg2576 mouse brains after injection of Cur -MNPs, and no plaques could be found in non-transgenic mice. Immunohistochemical examination of the mouse brains revealed that Cur-MNPs were co-localized with amyloid plaques. Thus, Cur-MNPs have the potential for non-invasive diagnosis of AD using MRI. (C) 2014 Elsevier Ltd. All rights reserved.
All Author(s) ListCheng KK, Chan PS, Fan SJ, Kwan SM, Yeung KL, Wang YXJ, Chow AHL, Wu EX, Baum L
Journal nameBiomaterials,Biomaterials
Volume Number44
Pages155 - 172
LanguagesEnglish-United Kingdom
KeywordsAlzheimer's disease; Amyloid plaques; Curcumin; Iron oxide nanoparticles; MRI; Tg2576 mice
Web of Science Subject CategoriesEngineering; Engineering, Biomedical; Materials Science; Materials Science, Biomaterials

Last updated on 2020-25-10 at 02:03