NMR investigation of DNA primer-template models: Structural insights into dislocation mutagenesis in DNA replication
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AbstractSlipped frameshift intermediates can occur when DNA polymerase slows or stalls at sites of DNA lesions. However, this phenomenon is much less common when unmodified DNA is replicated. In order to study the effect of templating bases on the alignment of primer-templates, NMR structural investigation has been performed on primer-template oligonucleotide models which mimic the situation that dNTP has just been incorporated opposite template. NMR evidence reveals the occurrence of misalignment when dGTP is incorporated opposite template T with a downstream nucleotide C. Depending on the template sequence, further extension of the primer can lead to realignment. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
All Author(s) ListChi LM, Lam SL
Journal nameFEBS Letters,FEBS Letters
Volume Number580
Issue Number27
Pages6496 - 6500
LanguagesEnglish-United Kingdom
Keywordsbulge; DNA; low fidelity polymerase; misalignment; NMR; primer-template
Web of Science Subject CategoriesBiochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS; Cell Biology; CELL BIOLOGY

Last updated on 2021-16-09 at 01:05